Please use this identifier to cite or link to this item:
Title: Automated morphological feature assessment for zebrafish embryo developmental toxicity screens
Author: Teixidó Condomines, Elisabet
Kießling, Tobias R.
Krupp, E.
Quevedo, Celia
Muriana, Arantza
Scholz, Stefan
Keywords: Embriologia
Peix zebra
Zebra danio
Issue Date: 1-Feb-2019
Publisher: Academic Press
Abstract: Detection of developmental phenotypes in zebrafish embryos typically involves a visual assessment and scoring of morphological features by an individual researcher. Subjective scoring could impact results and be of particular concern when phenotypic effect patterns are also used as a diagnostic tool to classify compounds. Here we introduce a quantitative morphometric approach based on image analysis of zebrafish embryos. A software called FishInspector was developed to detect morphological features from images collected using an automated system to position zebrafish embryos. The analysis was verified and compared with visual assessments of 3 participating laboratories using 3 known developmental toxicants (methotrexate, dexamethasone, and topiramate) and 2 negative compounds (loratadine and glibenclamide). The quantitative approach exhibited higher sensitivity and made it possible to compare patterns of effects with the potential to establish a grouping and classification of developmental toxicants. Our approach improves the robustness of phenotype scoring and reliability of assay performance and, hence, is anticipated to improve the predictivity of developmental toxicity screening using the zebrafish embryo.
Note: Versió postprint del document publicat a:
It is part of: Toxicological Sciences, 2019, vol. 167, num. 2, p. 438-449
Related resource:
ISSN: 1096-6080
Appears in Collections:Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)

Files in This Item:
File Description SizeFormat 
698535.pdf802.78 kBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons