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Title: Implementation of a functional endpoint to the zebrafish embryotoxicity test to evaluate craniofacial abnormalities
Author: Barenys Espadaler, Marta
Molins Raich, Anna
Amorós-Galicia, Lola
Flick, Burkhard
Gómez Catalán, Jesús
Keywords: Peix zebra
Mètodes experimentals
Zebra danio
Experimental methods
Issue Date: 30-Aug-2019
Publisher: Elsevier Ltd
Abstract: The inclusion of a read-out to detect functional consequences of craniofacial alterations in the zebrafish embryotoxicity test will allow to evaluate these alterations which are difficult to assess morphologically, and to detect alterations in cranial nerves functions leading to impairment of jaw movements. In this study we have established an ingestion test in zebrafish larvae younger than 120 hpf. To overcome the challenge of evaluating larvae which still do not present independent feeding behaviour, we have tested the ability of 72, 96 or 102 hpf larvae to ingest food mixed with fluorescent microspheres under several conditions (dark/light, with/without shaking) to find the best experimental set-up for the test. We have included the investigation of two substances as potential positive controls: ketoconazole and tricaine. Ketoconazole 10 μM exposure during development produced significant embryotoxic effects including a characteristic craniofacial alteration pattern consisting in impaired development of brain, nasal cavity, mouth opening and jaw, as well as a significant decrease in food intake. Tricaine exposure at 380 μM during the food availability period significantly decreased the food intake. The method proposed will be a useful alternative tool to animal testing to detect compounds inducing adverse effects on craniofacial development.
Note: Versió postprint del document publicat a:
It is part of: Toxicology in Vitro, 2019, vol. 61, p. 104638
Related resource:
ISSN: 0887-2333
Appears in Collections:Articles publicats en revistes (Institut de Recerca en Nutrició i Seguretat Alimentària (INSA·UB))
Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)

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