Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/167804
Title: | Transient exposure to miR-203 expands the differentiation capacity of pluripotent stem cells |
Author: | Salazar-Roa, María Trakala, Marianna Álvarez Fernández, Mónica Valdés Mora, Fátima Zhong, Cuiqing Muñoz, Jaime Yu, Yang Peters, Timothy J. Graña Castro, Osvaldo Serrano, Rosa Zapatero Solana, Elisabet Abad, María Lluisa Bueno, María José Gómez de Cedrón, Marta Fernández Piqueras, José Serrano Marugán, Manuel Blasco, María A. Wang, Da-Zhi Clark, Susan J. Izpisúa Belmonte, Juan Carlos Ortega, Sagrario Malumbre, Marcos |
Keywords: | Micro RNAs Cèl·lules mare Epigenètica MicroRNAs Stem cells Epigenetics |
Issue Date: | 2-Jul-2020 |
Publisher: | EMBO Press |
Abstract: | Full differentiation potential along with self‐renewal capacity is a major property of pluripotent stem cells (PSCs). However, the differentiation capacity frequently decreases during expansion of PSCs in vitro . We show here that transient exposure to a single microRNA, expressed at early stages during normal development, improves the differentiation capacity of already‐established murine and human PSCs. Short exposure to miR‐203 in PSCs (mi PSCs) induces a transient expression of 2C markers that later results in expanded differentiation potency to multiple lineages, as well as improved efficiency in tetraploid complementation and human–mouse interspecies chimerism assays. Mechanistically, these effects are at least partially mediated by direct repression of de novo DNA methyltransferases Dnmt3a and Dnmt3b, leading to transient and reversible erasure of DNA methylation. These data support the use of transient exposure to miR‐203 as a versatile method to reset the epigenetic memory in PSCs, and improve their effectiveness in regenerative medicine. |
Note: | Versió postprint del document publicat a: https://doi.org/10.15252/embj.2019104324 |
It is part of: | The EMBO Journal, 2020, e104324 |
URI: | http://hdl.handle.net/2445/167804 |
Related resource: | https://doi.org/10.15252/embj.2019104324 |
Appears in Collections: | Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
EMBOJ-2019-104324R1.pdf | 5.41 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.