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Title: A Novel Saliva-Based miRNA Signature for Colorectal Cancer Diagnosis
Author: Rapado González, Óscar
Majem, Blanca
Álvarez Castro, Ana
Díaz Peña, Roberto
Abalo, Alicia
Suárez Cabrera, Leticia
Gil Moreno, Antonio
Santamaría, Anna
López López, Rafael
Muinelo Romay, Laura
Suarez Cunqueiro, María Mercedes
Keywords: Càncer colorectal
Marcadors bioquímics
Colorectal cancer
Biochemical markers
Issue Date: 1-Dec-2019
Publisher: Mdpi
Abstract: Salivary microRNAs (miRNAs) are of high interest as diagnostic biomarkers for non-oral cancer. However, little is known about their value for colorectal cancer (CRC) detection. Our study aims to characterize salivary miRNAs in order to identify non-invasive markers for CRC diagnosis. The screening of 754 miRNAs was performed in saliva samples from 14 CRC and 10 healthy controls. The differential expressed miRNAs were validated by RT-qPCR in 51 CRC, 19 adenomas and 37 healthy controls. Receiver operating characteristic (ROC) curves and logistic regression models were performed to analyze the clinical value of these miRNAs. Twenty-two salivary miRNAs were significantly deregulated in CRC patients vs. healthy individuals (p < 0.05) in the discovery phase. From those, five upregulated miRNAs (miR-186-5p, miR-29a-3p, miR-29c-3p, miR-766-3p, and miR-491-5p) were confirmed to be significantly higher in the CRC vs. healthy group (p < 0.05). This five-miRNA signature showed diagnostic value (72% sensitivity, 66.67% specificity, AUC = 0.754) to detect CRC, which was even higher in combination with carcinoembryonic antigen (CEA) levels. Overall, after the first global characterization of salivary miRNAs in CRC, a five-miRNA panel was identified as a promising tool to diagnose this malignancy, representing a novel approach to detect cancer-associated epigenetic alterations using a non-invasive strategy.
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It is part of: Journal Of Clinical Medicine, 2019-12-01, Vol. 8, Issue 12
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Appears in Collections:Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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