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Title: | Exploring frequencies of circulating specific Th17 cells against myeloperoxidase and proteinase 3 in ANCA associated vasculitis |
Author: | Martínez Valenzuela, Laura Draibe, Juliana Quero Ramos, Maria Fulladosa, Xavier Cruzado, Josep Ma. Bestard Matamoros, Oriol Torras Ambròs, Joan |
Keywords: | Vasculitis Diagnòstic Cèl·lules Immunologia Vasculitis Diagnosis Cells Immunology |
Issue Date: | 20-Nov-2019 |
Publisher: | MDPI |
Abstract: | Background: the role of the T helper 17 (Th17) cell subset in anti-neutrophil cytoplasm antibodies (ANCA) associated vasculitis (AAV) is controversial. We hypothesized that a specific Th17 response to myeloperoxidase (MPO) or proteinase 3 (PR3) is detectable in AAV patients and is different among the disease phases. Methods: we analyzed 43 AAV patients with renal involvement (21 acute and 22 remission patients), and 12 healthy controls. Peripheral blood mononuclear cells (PBMCs) were cultured with PR3/MPO over 48 h. Thereafter, frequencies of MPO/PR3-specific Th17 cells were assessed using an enzyme-linked immunosorbent spot (ELISpot) assay. Supernatant IL-17 concentration was quantified using ELISA. Finally, specific Th17 response after depletion of T regulatory lymphocytes (T-regs) in some remission patients was compared to the non T-reg-depleted response. Results: specific Th17 cell number was higher in acute patients compared to remission (p = 0.004). Specific Th17 cell number performed well in the disease activity detection (ROC curve area under the curve (AUC) = 0.87; p = 0.0001) with an optimal cut-off of 6 spots/million. Patients above this cut-off showed higher serum creatinine (p = 0.004), C-reactive protein (CRP) (p = 0.001) and ANCA titer (p = 0.032). Supernatant IL-17 concentration was higher in acute patients compared to remission (p = 0.035) and did not normalize to healthy control levels (p = 0.01). Conclusions: a specific Th17 cell response is present in AAV patients. This response is more pronounced in the acute phase, but persists in remission. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/ijms20235820 |
It is part of: | International Journal of Molecular Sciences, 2019, vol. 20, num. 23, p. 5820 |
URI: | http://hdl.handle.net/2445/168223 |
Related resource: | https://doi.org/10.3390/ijms20235820 |
ISSN: | 1661-6596 |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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