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http://hdl.handle.net/2445/168441
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DC Field | Value | Language |
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dc.contributor.author | Berbel, Dámaris | - |
dc.contributor.author | Cámara, Jordi | - |
dc.contributor.author | García, Ernesto | - |
dc.contributor.author | Tubau, Fe | - |
dc.contributor.author | Guérin, François | - |
dc.contributor.author | Giard, Jean-Christophe | - |
dc.contributor.author | Domínguez Luzón, Ma. Ángeles (María Ángeles) | - |
dc.contributor.author | Cattoir, Vincent | - |
dc.contributor.author | Ardanuy Tisaire, María Carmen | - |
dc.date.accessioned | 2020-07-13T09:39:36Z | - |
dc.date.available | 2020-11-01T06:10:22Z | - |
dc.date.issued | 2019-11-01 | - |
dc.identifier.issn | 0924-8579 | - |
dc.identifier.uri | http://hdl.handle.net/2445/168441 | - |
dc.description.abstract | A lincosamide-resistant and macrolide-susceptible phenotype has not been described to date in Streptococcus pyogenes [group A streptococcus (GAS)]. The aim of this study was to characterize a GAS isolate susceptible to macrolides but resistant to lincosamide, streptogramin A and pleuromutilin antibiotics. Antimicrobial susceptibility was tested using the microdilution broth method and the resistance phenotype was tested by D-test. The GAS2887HUB isolate was subjected to whole-genome sequencing. The isolate showed a positive Gots' test (clindamycin inactivation). Whole-genome sequencing revealed that the strain was ST10 and emm93, and had five resistance genes [lnu(B), ant(6)-Ia, aph(3')-III, tet(M) and dfrG]. The tet(M) gene was located in a Tn916-like transposon. The lsa(E)-lnu(B)-containing sequence (inserted downstream of the rumA gene) was formed by a 39.6-kb prophage, followed by a gene cluster encoding aminoglycoside-streptothricin resistance [ant(6)Ia-sat4-aph(3')III] and lsa(E)-lnu(B) genes. This structure was not transferred by conjugation. This study identified a new genetic element carrying a determinant of lincosamide resistance in a GAS. Further molecular epidemiological surveys are needed to determine the prevalence of this mechanism of resistance in GAS. | - |
dc.format.extent | 5 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.ijantimicag.2019.08.019 | - |
dc.relation.ispartof | International Journal of Antimicrobial Agents, 2019, vol. 54, num. 5, p. 647-651 | - |
dc.relation.uri | https://doi.org/10.1016/j.ijantimicag.2019.08.019 | - |
dc.rights | cc-by-nc-nd (c) Elsevier B.V., 2019 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Fenotip | - |
dc.subject.classification | Estreptococs | - |
dc.subject.classification | Antibiòtics | - |
dc.subject.other | Phenotype | - |
dc.subject.other | Streptococcus | - |
dc.subject.other | Antibiotics | - |
dc.title | A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 693755 | - |
dc.date.updated | 2020-07-13T09:39:36Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 31476434 | - |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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693755.pdf | 449.61 kB | Adobe PDF | View/Open |
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