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Title: Chemical Modification of Microcin J25 Reveals New Insights on the Stereospecific Requirements for Antimicrobial Activity
Author: Martín Gómez, Helena
Jorba, Marta
Albericio Palomera, Fernando
Viñas, Miquel
Tulla-Puche, Judit
Keywords: Pèptids
Issue Date: 2-Oct-2019
Publisher: Mdpi
Abstract: In this study, microcin J25, a potent antimicrobial lasso peptide that acts on Gram-negative bacteria, was subjected to a harsh treatment with a base in order to interrogate its stability and mechanism of action and explore its structure-activity relationship. Despite the high stability reported for this lasso peptide, the chemical treatment led to the detection of a new product. Structural studies revealed that this product retained the lasso topology, but showed no antimicrobial activity due to the epimerization of a key residue for the activity. Further microbiological assays also demonstrated that it showed a high synergistic effect with colistin.
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It is part of: International Journal Of Molecular Sciences, 2019-10-02, Vol. 20, num. 20, p. 5152
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Appears in Collections:Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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