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Title: Global hyperactivation of enhancers stabilizes human and mouse naïve pluripotency through inhibition of CDK8/19 Mediator kinases
Author: Lynch, Cian J.
Bernad, Raquel
Martínez Val, Ana
Shahbazi, Marta N.
Nóbrega Pereira, Sandrina
Calvo, Isabel
Blanco Aparicio, Carmen
Tarantino, Carolina
Garreta, Elena
Richart Ginés, Laia
Alcazar, Noelia
Graña Castro, Osvaldo
Gómez López, Gonzalo
Aksoy, Irène
Muñoz-Martín, Maribel
Martinez, Sonia
Ortega, Sagrario
Prieto, Susana
Simboeck, Elisabeth
Camasses, Alain
Stephan-Otto Attolini, Camille
Fernandez, Agustin F.
Sierra, Marta I.
Fraga, Mario F.
Pastor, Joaquin
Fisher, Daniel
Montserrat, Núria
Savatier, Pierre
Muñoz, Javier
Zernicka-Goetz, Magdalena
Serrano Marugán, Manuel
Keywords: Cèl·lules mare
Stem cells
Issue Date: 28-Sep-2020
Publisher: Springer Nature
Abstract: Pluripotent stem cells (PSCs) transition between cell states in vitro and reflect developmental changes in the early embryo. PSCs can be stabilized in the naïve state by blocking extracellular differentiation stimuli, particularly FGF-MEK signaling. Here, we report that multiple features of the naïve state in human and mouse PSCs can be recapitulated without affecting FGF-MEK-signaling or global DNA methylation. Mechanistically, chemical inhibition of CDK8 and CDK19 kinases removes their ability to repress the Mediator complex at enhancers. Thus CDK8/19 inhibition increases Mediator-driven recruitment of RNA Pol II to promoters and enhancers. This efficiently stabilizes the naïve transcriptional program and confers resistance to enhancer perturbation by BRD4 inhibition. Moreover, naïve pluripotency during embryonic development coincides with a reduction in CDK8/19. We conclude that global hyperactivation of enhancers drives naïve pluripotency, and this can be achieved in vitro by inhibiting CDK8/19 kinase activity. These principles may apply to other contexts of cellular plasticity.
Note: Versió postprint del document publicat a:
It is part of: Nature Cell Biology, 2020, vol. 22, pàg. 1223-1238
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ISSN: 1465-7392
Appears in Collections:Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

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