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Title: | Ex vivo culture of lesional psoriasis skin for pharmacological testing |
Author: | Tiirikainen, Minna Lund Woetmann, Anders Norsgaard, Hanne Santamaria Babí, Luis F. Lovato, Paola |
Keywords: | Psoriasi Immunoregulació Psoriasis Immunoregulation |
Issue Date: | 27-Dec-2019 |
Publisher: | Elsevier |
Abstract: | Background: Psoriasis is a chronic, inflammatory skin disorder resulting from a complex interplay between immune and skin cells via release of soluble mediators. While a lot is known about the molecular mechanisms behind psoriasis pathogenesis, there is still a need for preclinical research models that accuratelyreplicate the disease. Objective: This study aimed to develop and characterize ex vivo culture of psoriasis skin as a model for pharmacological testing, where the immunological events of psoriasis can be followed. Methods: Full thickness punch biopsies of lesional psoriasis skin were cultured in submerged conditions up to 144 h followingin situ T cell stimulation with rhIL-23 and anti-CD3 and anti-CD28 antibodies. The Tcell mediated skin inflammation was assessed by gene and protein l analysis for a panel of inflammatory mediators. Tissue integrity and morphology were evaluated by histological analysis. Results: T cell stimulation resulted in functional and psoriasis specificin situ activation of T cells. The expression levels of most of the proinflammatory mediators related to both immune and skin cells were comparable to these in freshly isolated tissue at 48 and 96 h of culture. Tissue integrity and morphology were sustained up to 96 h. Treatment with a corticosteroid reduced the expression of several proinflammatory cytokines and chemokines, whereas anti-IL-17A antibody treatment reduced the expression of the IL-17A downstream markers IL-8 and DEFB4. Conclusion: By preserving keyimmunopathological mechanisms of psoriasis, ex vivo culture of psoriasis skin can be used for the investigation of inflammatory processes of psoriasis and for preclinical drug discovery research. |
Note: | Reproducció del document publicat a: https://doi.org/10.1016/j.jdermsci.2019.12.010 |
It is part of: | Journal of Dermatological Science, 2019, vol. 97, num. 2, p. 109-116 |
URI: | http://hdl.handle.net/2445/171181 |
Related resource: | https://doi.org/10.1016/j.jdermsci.2019.12.010 |
ISSN: | 0923-1811 |
Appears in Collections: | Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
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