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http://hdl.handle.net/2445/171181
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DC Field | Value | Language |
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dc.contributor.author | Tiirikainen, Minna Lund | - |
dc.contributor.author | Woetmann, Anders | - |
dc.contributor.author | Norsgaard, Hanne | - |
dc.contributor.author | Santamaria Babí, Luis F. | - |
dc.contributor.author | Lovato, Paola | - |
dc.date.accessioned | 2020-10-13T14:23:03Z | - |
dc.date.available | 2020-10-13T14:23:03Z | - |
dc.date.issued | 2019-12-27 | - |
dc.identifier.issn | 0923-1811 | - |
dc.identifier.uri | http://hdl.handle.net/2445/171181 | - |
dc.description.abstract | Background: Psoriasis is a chronic, inflammatory skin disorder resulting from a complex interplay between immune and skin cells via release of soluble mediators. While a lot is known about the molecular mechanisms behind psoriasis pathogenesis, there is still a need for preclinical research models that accuratelyreplicate the disease. Objective: This study aimed to develop and characterize ex vivo culture of psoriasis skin as a model for pharmacological testing, where the immunological events of psoriasis can be followed. Methods: Full thickness punch biopsies of lesional psoriasis skin were cultured in submerged conditions up to 144 h followingin situ T cell stimulation with rhIL-23 and anti-CD3 and anti-CD28 antibodies. The Tcell mediated skin inflammation was assessed by gene and protein l analysis for a panel of inflammatory mediators. Tissue integrity and morphology were evaluated by histological analysis. Results: T cell stimulation resulted in functional and psoriasis specificin situ activation of T cells. The expression levels of most of the proinflammatory mediators related to both immune and skin cells were comparable to these in freshly isolated tissue at 48 and 96 h of culture. Tissue integrity and morphology were sustained up to 96 h. Treatment with a corticosteroid reduced the expression of several proinflammatory cytokines and chemokines, whereas anti-IL-17A antibody treatment reduced the expression of the IL-17A downstream markers IL-8 and DEFB4. Conclusion: By preserving keyimmunopathological mechanisms of psoriasis, ex vivo culture of psoriasis skin can be used for the investigation of inflammatory processes of psoriasis and for preclinical drug discovery research. | - |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1016/j.jdermsci.2019.12.010 | - |
dc.relation.ispartof | Journal of Dermatological Science, 2019, vol. 97, num. 2, p. 109-116 | - |
dc.relation.uri | https://doi.org/10.1016/j.jdermsci.2019.12.010 | - |
dc.rights | cc-by-nc-nd (c) Tiirikainen et al., 2019 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es | - |
dc.source | Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) | - |
dc.subject.classification | Psoriasi | - |
dc.subject.classification | Immunoregulació | - |
dc.subject.other | Psoriasis | - |
dc.subject.other | Immunoregulation | - |
dc.title | Ex vivo culture of lesional psoriasis skin for pharmacological testing | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 695091 | - |
dc.date.updated | 2020-10-13T14:23:03Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 31948839 | - |
Appears in Collections: | Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
Files in This Item:
File | Description | Size | Format | |
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695091.pdf | 2.65 MB | Adobe PDF | View/Open |
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