Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/171219
Title: Semen miRNAs Contained in Exosomes as Non-Invasive Biomarkers for Prostate Cancer Diagnosis
Author: Barceló, Maria
Castells, Manel
Bassas, Lluís
Vigues, Francesc
Larriba, Sara
Keywords: Càncer de pròstata
Marcadors bioquímics
Semen
Prostate cancer
Biochemical markers
Semen
Issue Date: 24-Jan-2019
Publisher: Nature Publishing Group
Abstract: Although it is specific for prostatic tissue, serum prostate-specific antigen (PSA) screening has resulted in an over-diagnosis of prostate cancer (PCa) and many unnecessary biopsies of benign disease due to a well-documented low cancer specificity, thus improvement is required. We profiled the expression level of miRNAs contained in semen exosomes from men with moderately increased PSA levels to assess their usefulness, either alone or in addition to PSA marker, as non-invasive biomarkers, for the early efficient diagnosis and prognosis of PCa. An altered miRNA expression pattern was found by a high throughput profiling analysis in PCa when compared with healthy individuals (HCt) exosomal semen samples. The presence of vasectomy was taken into account for the interpretation of results. Fourteen miRNAs were selected for miRNA validation as PCa biomarkers in a subsequent set of semen samples. In this explorative study, we describe miRNA-based models, which included miRNA expression values together with PSA levels, that increased the classification function of the PSA screening test with diagnostic and/or prognostic potential: [PSA miR-142-3p miR-142-5p miR-223-3p] model (AUC:0,821) to discriminate PCa from BPH (Sn:91,7% Sp:42,9% vs Sn:100%Sp:14,3%); and [PSA miR-342-3p +/- miR-374b-5p] model (AUC: 0,891) to discriminate between GS >= 7 tumours and men presenting PSA >= 4 ng/ml with no cancer or GS6 tumours (Sn:81,8% Sp:95% vs Sn:54,5% Sp:90%). The pathway analysis of predicted miRNA target genes supports a role for these miRNAs in PCa aetiology and/or progression. Our study shows semen exosome miRNA-based models as molecular biomarkers with the potential to improve PCa diagnosis/prognosis efficiency. As the next step, further prospective studies on larger cohorts of patients are required to validate the diagnostic and/or prognostic role of the miRNA panel before it could be adopted into clinical practice.
Note: Reproducció del document publicat a: https://doi.org/10.1038/s41598-019-50172-6
It is part of: Scientific Reports, 2019, vol. 9, p. 13772
URI: http://hdl.handle.net/2445/171219
Related resource: https://doi.org/10.1038/s41598-019-50172-6
Appears in Collections:Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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