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http://hdl.handle.net/2445/171333
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DC Field | Value | Language |
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dc.contributor.author | Garcia Puig, Anna | - |
dc.contributor.author | Mosquera Mayo, José Luis | - |
dc.contributor.author | Jiménez Delgado, Senda | - |
dc.contributor.author | Garcia Pastor, Cristina | - |
dc.contributor.author | Jorba, Ignasi | - |
dc.contributor.author | Navajas Navarro, Daniel | - |
dc.contributor.author | Canals, Francesc | - |
dc.contributor.author | Raya Chamorro, Ángel | - |
dc.date.accessioned | 2020-10-19T08:31:25Z | - |
dc.date.available | 2020-10-19T08:31:25Z | - |
dc.date.issued | 2019-09 | - |
dc.identifier.issn | 1535-9476 | - |
dc.identifier.uri | http://hdl.handle.net/2445/171333 | - |
dc.description.abstract | Adult zebrafish, in contrast to mammals, are able to regenerate their hearts in response to injury or experimental amputation. Our understanding of the cellular and molecular bases that underlie this process, although fragmentary, has increased significantly over the last years. However, the role of the extracellular matrix (ECM) during zebrafish heart regeneration has been comparatively rarely explored. Here, we set out to characterize the ECM protein composition in adult zebrafish hearts, and whether it changed during the regenerative response. For this purpose, we first established a decellularization protocol of adult zebrafish ventricles that significantly enriched the yield of ECM proteins. We then performed proteomic analyses of decellularized control hearts and at different times of regeneration. Our results show a dynamic change in ECM protein composition, most evident at the earliest (7 days postamputation) time point analyzed. Regeneration associated with sharp increases in specific ECM proteins, and with an overall decrease in collagens and cytoskeletal proteins. We finally tested by atomic force microscopy that the changes in ECM composition translated to decreased ECM stiffness. Our cumulative results identify changes in the protein composition and mechanical properties of the zebrafish heart ECM during regeneration. | - |
dc.format.extent | 11 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1074/mcp.RA118.001193 | - |
dc.relation.ispartof | Molecular & Cellular Proteomics, 2019, vol. 18, num. 9, p. 1745-1755 | - |
dc.relation.uri | https://doi.org/10.1074/mcp.RA118.001193 | - |
dc.rights | cc by (c) Garcia Puig et al., 2019 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | - |
dc.source | Articles publicats en revistes (Biomedicina) | - |
dc.subject.classification | Malalties cardiovasculars | - |
dc.subject.classification | Models animals en la investigació | - |
dc.subject.classification | Biologia del desenvolupament | - |
dc.subject.other | Cardiovascular diseases | - |
dc.subject.other | Animal models in research | - |
dc.subject.other | Developmental biology | - |
dc.title | Proteomics Analysis of Extracellular Matrix Remodeling During Zebrafish Heart Regeneration | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 692245 | - |
dc.date.updated | 2020-10-19T08:31:25Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 31221719 | - |
Appears in Collections: | Articles publicats en revistes (Biomedicina) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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File | Description | Size | Format | |
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692245.pdf | 2.23 MB | Adobe PDF | View/Open |
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