Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/171335
Title: Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety)
Author: Sánchez Garcés, Ma. Ángeles
Vinuesa Aumedes, Teresa
Ciurana, Joan
Viñas, Miquel
Keywords: Implants dentals
Esterilització
Dental implants
Sterilization
Issue Date: 1-Sep-2019
Publisher: Medicina Oral SL
Abstract: BACKGROUND: The reuse of implant healing abutments is common in dental practice. Effective elimination of bacteria and viruses is accomplished by conventional sterilization. The aim of this work was to explore the eventual survival of microorganisms on sterilized healing abutments and to rule out the presence of transmissible organic material after standard procedures. MATERIAL AND METHODS: A total of 55 healing abutments previously used in patients will be washed and sterilized in a steam autoclave at 121 C for 15 min. Each healing abutment will be cultured in Brain Heart Infusion broth (BHI) under strict aseptic conditions. Besides, two control groups will be included: one of 3 unused healing abutments, and the other of just medium. After 10 days at 37°C under a 5% CO2 100 µl of the broth will be plated on solid media (Brain Infusion Agar, BHIA) and Columbia Blood agar to test for sterility. The remaining volume will be centrifuged, the sediment fixed, and a Gram stain performed to discard the presence of non-cultivable microorganisms. Moreover, to determine the presence of remaining organic material after the cleaning and sterilizing treatments, the bioburden will be determined by measuring total organic carbon (TOC) in another 10 previously used healing abutments, cleaned and sterilized, that will be submerged in Milli-Q water and sonicated. RESULTS: No bacterial growth was detected on any of the 58 cultured abutments, indicating that the sterilization was completely satisfactory in terms of removal of live bacteria or spores. Nevertheless, significant amounts of organic carbon may still be recovered (up to 125,31 µg/abutment) after they have been sterilized. CONCLUSIONS: Significant amounts of the bioburden remained adhered to the surfaces in spite of the cleaning and sterilization procedures. Taking into account our results and data from other authors, the presence of infectious particles on the reused healing abutments such as prions cannot be ruled out.
Note: Reproducció del document publicat a: https://doi.org/10.4317/medoral.22967
It is part of: Medicina Oral, Patología Oral y Cirugía Bucal, 2019, vol. 24, num. 5, p. e583-e587
URI: http://hdl.handle.net/2445/171335
Related resource: https://doi.org/10.4317/medoral.22967
ISSN: 1698-4447
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Odontoestomatologia)

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