Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/171541
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dc.contributor.authorFernández Santiago, Rubén-
dc.contributor.authorMerkel, Angelika-
dc.contributor.authorCastellano, Giancarlo-
dc.contributor.authorHeath, Simon-
dc.contributor.authorRaya Chamorro, Ángel-
dc.contributor.authorTolosa, Eduardo-
dc.contributor.authorMarti, Maria Jose-
dc.contributor.authorConsiglio, Antonella-
dc.contributor.authorEzquerra, Mario-
dc.date.accessioned2020-10-27T10:53:08Z-
dc.date.available2020-10-27T10:53:08Z-
dc.date.issued2019-07-23-
dc.identifier.issn1868-7075-
dc.identifier.urihttp://hdl.handle.net/2445/171541-
dc.description.abstractBackground: Parkinson's disease (PD) is characterized by the loss of midbrain dopaminergic neurons (DAn). Previously, we described the presence of DNA hyper- and hypo-methylation alterations in induced pluripotent stem cells (iPSC)-derived DAn from PD patients using the Illumina 450K array which prominently covers gene regulatory regions. Methods: To expand and contextualize previous findings, we performed the first whole-genome DNA bisulfite sequencing (WGBS) using iPSC-derived DAn from representative PD subjects: one sporadic PD (sPD) patient, one monogenic LRRK2-associated PD patient (L2PD), and one control. Results: At the whole-genome level, we detected global DNA hyper-methylation in the PD which was similarly spread across the genome in both sPD and L2PD and mostly affected intergenic regions. Conclusion: This study implements previous epigenetic knowledge in PD at a whole genome level providing the first comprehensive and unbiased CpG DNA methylation data using iPSC-derived DAn from PD patients. Our results indicate that DAn from monogenic or sporadic PD exhibit global DNA hyper-methylation changes. Findings from this exploratory study are to be validated in further studies analyzing other PD cell models and patient tissues.-
dc.format.extent7 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherBioMed Central-
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1186/s13148-019-0701-6-
dc.relation.ispartofClinical Epigenetics, 2019, vol. 11-
dc.relation.urihttps://doi.org/10.1186/s13148-019-0701-6-
dc.rightscc-by (c) Fernández Santiago, Rubén et al., 2019-
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es-
dc.sourceArticles publicats en revistes (Patologia i Terapèutica Experimental)-
dc.subject.classificationMalaltia de Parkinson-
dc.subject.classificationADN-
dc.subject.classificationMetilació-
dc.subject.otherParkinson's disease-
dc.subject.otherDNA-
dc.subject.otherMethylation-
dc.titleWhole-genome DNA hyper-methylation in iPSC-derived dopaminergic neurons from Parkinson's disease patients-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.identifier.idgrec697533-
dc.date.updated2020-10-27T10:53:08Z-
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/311736/EU//PD-HUMMODEL-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
dc.identifier.pmid31337434-
Appears in Collections:Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Institut de Biomedicina (IBUB))
Articles publicats en revistes (Patologia i Terapèutica Experimental)
Publicacions de projectes de recerca finançats per la UE
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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