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http://hdl.handle.net/2445/171541
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DC Field | Value | Language |
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dc.contributor.author | Fernández Santiago, Rubén | - |
dc.contributor.author | Merkel, Angelika | - |
dc.contributor.author | Castellano, Giancarlo | - |
dc.contributor.author | Heath, Simon | - |
dc.contributor.author | Raya Chamorro, Ángel | - |
dc.contributor.author | Tolosa, Eduardo | - |
dc.contributor.author | Marti, Maria Jose | - |
dc.contributor.author | Consiglio, Antonella | - |
dc.contributor.author | Ezquerra, Mario | - |
dc.date.accessioned | 2020-10-27T10:53:08Z | - |
dc.date.available | 2020-10-27T10:53:08Z | - |
dc.date.issued | 2019-07-23 | - |
dc.identifier.issn | 1868-7075 | - |
dc.identifier.uri | http://hdl.handle.net/2445/171541 | - |
dc.description.abstract | Background: Parkinson's disease (PD) is characterized by the loss of midbrain dopaminergic neurons (DAn). Previously, we described the presence of DNA hyper- and hypo-methylation alterations in induced pluripotent stem cells (iPSC)-derived DAn from PD patients using the Illumina 450K array which prominently covers gene regulatory regions. Methods: To expand and contextualize previous findings, we performed the first whole-genome DNA bisulfite sequencing (WGBS) using iPSC-derived DAn from representative PD subjects: one sporadic PD (sPD) patient, one monogenic LRRK2-associated PD patient (L2PD), and one control. Results: At the whole-genome level, we detected global DNA hyper-methylation in the PD which was similarly spread across the genome in both sPD and L2PD and mostly affected intergenic regions. Conclusion: This study implements previous epigenetic knowledge in PD at a whole genome level providing the first comprehensive and unbiased CpG DNA methylation data using iPSC-derived DAn from PD patients. Our results indicate that DAn from monogenic or sporadic PD exhibit global DNA hyper-methylation changes. Findings from this exploratory study are to be validated in further studies analyzing other PD cell models and patient tissues. | - |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1186/s13148-019-0701-6 | - |
dc.relation.ispartof | Clinical Epigenetics, 2019, vol. 11 | - |
dc.relation.uri | https://doi.org/10.1186/s13148-019-0701-6 | - |
dc.rights | cc-by (c) Fernández Santiago, Rubén et al., 2019 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Malaltia de Parkinson | - |
dc.subject.classification | ADN | - |
dc.subject.classification | Metilació | - |
dc.subject.other | Parkinson's disease | - |
dc.subject.other | DNA | - |
dc.subject.other | Methylation | - |
dc.title | Whole-genome DNA hyper-methylation in iPSC-derived dopaminergic neurons from Parkinson's disease patients | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 697533 | - |
dc.date.updated | 2020-10-27T10:53:08Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/311736/EU//PD-HUMMODEL | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 31337434 | - |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Institut de Biomedicina (IBUB)) Articles publicats en revistes (Patologia i Terapèutica Experimental) Publicacions de projectes de recerca finançats per la UE Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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697533.pdf | 739.03 kB | Adobe PDF | View/Open |
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