Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/171719
Title: Urolithins Are the Main Urinary Microbial-Derived Phenolic Metabolites Discriminating a Moderate Consumption of Nuts in FreeLiving Subjects with Diagnosed Metabolic Syndrome
Author: Tulipani, Sara
Urpí Sardà, Mireia
Garcia Villalba, Rocio
Rabassa Bonet, Montserrat
Lopez-Uriarte, Patricia
Bulló, Mònica
Jáuregui Pallarés, Olga
Tomas-Barberan, F. A. (Francisco A.)
Salas Salvadó, Jordi
Espín, Juan Carlos
Andrés Lacueva, Ma. Cristina
Keywords: Polifenols
Fenols
Fruita seca
Síndrome metabòlica
Microbiota
Polyphenols
Phenols
Dried fruit
Metabolic syndrome
Microbiota
Issue Date: 12-Sep-2012
Publisher: American Chemical Society
Abstract: Walnuts ( Juglans regia L.), hazelnuts ( Corylus avellana L.), and almonds ( Prunus dulcis Mill.) are rich sources of ellagitannins and proanthocyanidins. Gut microbiota plays a crucial role in modulating the bioavailability of these high molecular weight polyphenols. However, to date there are no studies evaluating the capacity to produce nut phenolic metabolites in subjects with metabolic syndrome (MetS), a pathology associated with an altered gut bacterial diversity. This study applied a LC-MS targeted approach to analyze the urinary excretion of nut phenolic metabolites in MetS subjects following 12 weeks of nut consumption, compared to sex- and age-matched individuals given a nut-free control diet. Metabolites were targeted in both hydrolyzed and nonhydrolyzed urine by LC-PDA-QqQ-MS/MS analysis, and identification of metabolites lacking available standards was confirmed by LC-ESI-ITD-FT-MS. Ellagitannin-derived urolithins A and B significantly increased after the nut-enriched-diet, urolithins C and D were also detected, and a complex combination of urolithin-conjugated forms was observed in nonhydrolyzed urine, confirming an extensive phase II metabolism after absorption. In contrast, no significant increases in proanthocyanidin microbial metabolites were observed in urine following nut consumption. Because the intestinal microbiota of the subjects in this study could catabolize ellagitannins into a wide range of urolithins, further research is strongly warranted on the in vivo potential of these microbial metabolites in reducing cardiometabolic risk.
Note: Versió postprint del document publicat a:
It is part of: Journal of Agricultural and Food Chemistry, 2012, vol. 60, num. 36, p. 8930-8940
URI: http://hdl.handle.net/2445/171719
ISSN: 0021-8561
Appears in Collections:Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)

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