Please use this identifier to cite or link to this item:
Title: Evaluation of the effects of acetylcholinesterase inhibitors in the zebrafish touch-evoked response: quantitative vs. qualitative assessment
Author: Guzman, Laura
Besa, Gisela
Linares, Daniela
González, Lara
Pont Masanet, Caterina
Bartolini, Manuela
Haigis, Ann-Cathrin
Legradi, Jessica
Muñoz-Torrero López-Ibarra, Diego
Gómez Catalán, Jesús
Barenys Espadaler, Marta
Keywords: Malaltia d'Alzheimer
Peix zebra
Alzheimer's disease
Zebra danio
Issue Date: 28-Oct-2020
Publisher: Springer Nature
Abstract: Background: The difficulty of finding new treatments for neurological diseases with great impact in our society like Alzheimer's disease can be ascribed in part to the complexity of the nervous system and the lack of quick and costeffective screening tools. Such tools could not only help to identify potential novel treatments, but could also be used to test environmental contaminants for their potential to cause neurotoxicity. It has been estimated that 5-10% of the anthropogenic chemicals are developmental neurotoxic (DNT) and exposure to DNT compounds has been linked to several neurological diseases. Within this study we were testing the applicability of a quick and cost-effective behavioural test using zebrafish embryos: the touch-evoked response assay, in this case, an assay evaluating the swimming response to a tap in the tail. Two acetylcholinesterase (AChE) inhibitors positive controls (paraoxon and huprine Y), as well as 10 huprine-derivative compounds were tested and the results were evaluated using 2 different methods, a quantitative and a qualitative one. Results: We could show that the methodology presented is able to detect behavioural effects of AChE inhibitors. A good correlation between the results obtained with the quantitative and the qualitative method was obtained (R2 = 0.84). Conclusions: Our proposed method enables combination of screening for new drugs with toxicity screening in a whole embryo model alternative to animal experimentation, thereby merging 2 drug development steps into one.
Note: Reproducció del document publicat a:
It is part of: Environmental Sciences Europe, 2020, vol. 32, p. 145
Related resource:
ISSN: 2190-4715
Appears in Collections:Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)

Files in This Item:
File Description SizeFormat 
704116.pdf1.99 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons