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http://hdl.handle.net/2445/171988
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DC Field | Value | Language |
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dc.contributor.author | Hueso Val, Miguel | - |
dc.contributor.author | Cruzado, Josep Ma. | - |
dc.contributor.author | Torras Ambròs, Joan | - |
dc.contributor.author | Navarro, Estanis | - |
dc.date.accessioned | 2020-11-11T14:21:32Z | - |
dc.date.available | 2020-11-11T14:21:32Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 2073-4425 | - |
dc.identifier.uri | http://hdl.handle.net/2445/171988 | - |
dc.description.abstract | Background: CD34+ Endothelial Progenitor Cells (EPCs) play an important role in the recovery of injured endothelium and contribute to atherosclerosis (ATH) pathogenesis. Previously we described a potential atherogenic role for miR-125 that we aimed to confirm in this work. Methods: Microarray hybridization, TaqMan Low Density Array (TLDA) cards, qPCR, and immunohistochemistry (IHC) were used to analyze expression of the miRNAs, proteins and transcripts here studied. Results: Here we have demonstrated an increase of resident CD34-positive cells in the aortic tissue of human and mice during ATH progression, as well as the presence of clusters of CD34-positive cells in the intima and adventitia of human ATH aortas. We introduce miR-351, which share the seed sequence with miR-125, as a potential effector of CD34. We show a splicing event at an internal/cryptic splice site at exon 8 of the murine Cd34 gene (exonic-switch) that would regulate the differential accession of miRNAs (including miR-125) to the coding region or to the 3'UTR of Cd34. Conclusions: We introduce new potential mediators of ATH progression (CD34 cell-clusters, miR-351), and propose a new mechanism of miRNA action, linked to a cryptic splicing site in the target-host gene, that would regulate the differential accession of miRNAs to their cognate binding sites. | - |
dc.format.extent | 16 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | MDPI | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3390/genes10010070 | - |
dc.relation.ispartof | Genes, 2019, vol. 10 | - |
dc.relation.uri | https://doi.org/10.3390/genes10010070 | - |
dc.rights | cc-by (c) Hueso Val, Miguel et al., 2019 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Clíniques) | - |
dc.subject.classification | Arterioesclerosi | - |
dc.subject.classification | Etiologia | - |
dc.subject.other | Arteriosclerosis | - |
dc.subject.other | Etiology | - |
dc.title | An exonic switch regulates differential accession of microRNAs to the Cd34 transcript in aterosclerosis progression | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 700035 | - |
dc.date.updated | 2020-11-11T14:21:32Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 30669689 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
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700035.pdf | 2.79 MB | Adobe PDF | View/Open |
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