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Title: Discovery of novel DNA methylation biomarkers for non‐invasive sporadic breast cancer detection in the Latino population
Author: Cappetta, Mónica
Fernandez, Lucía
Brignoni, Lucía
Artagaveytia, Nora
Bonilla, Carolina
López de Heredia, Miguel
Esteller, Manel
Bertoni, Bernardo
Berdasco, María
Keywords: ADN
Càncer de mama
Breast cancer
Issue Date: 3-Nov-2020
Publisher: Elsevier
Abstract: Human diversity is one of the main pitfalls in the development of robust worldwide biomarkers in oncology. Epigenetic variability across human populations are associated with different genetic backgrounds, as well as variable lifestyles and environmental exposures, each of which should be investigated. To identify potential non-invasive biomarkers of sporadic breast cancer in the Uruguayan population, we studied genome-wide DNA methylation using Illumina methylation arrays in leukocytes of 22 women with sporadic breast cancer and 10 healthy women in a case-control study. We described a panel of 38 differentially methylated CpG positions that was able to cluster breast cancer patients and controls, and that also recapitulated methylation differences in 12 primary breast tumors and their matched normal breast tissue. Moving forward we simplified the detection method to improve its applicability in a clinical setting, and used an independent well-characterized cohort of 80 leukocyte DNA samples from breast cancer patients and 80 healthy controls to validate methylation results at specific cancer-related genes. Our investigations identified methylation at CYFIP1 as a novel epigenetic biomarker candidate for sporadic breast cancer in the Uruguayan population. These results provide a proof-of-concept for the design of larger studies aimed at validating biomarker panels for the Latin American population.
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It is part of: Molecular Oncology, 2020
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ISSN: 1574-7891
Appears in Collections:Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Ciències Fisiològiques)

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