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Title: | Nr2e3 functional domain ablation by CRISPR-Cas9D10A identifies a new isoform and generates retinitis pigmentosa and enhanced S-cone syndrome models |
Author: | Aísa-Marín, Izarbe López-Iniesta, M.José Milla, Santiago Lillo, Jaume Navarro Brugal, Gemma de la Villa, Pedro Marfany i Nadal, Gemma |
Keywords: | Malalties de la retina Retinal diseases |
Issue Date: | Oct-2020 |
Publisher: | Elsevier |
Abstract: | Mutations in NR2E3 cause retinitis pigmentosa (RP) and enhanced S-cone syndrome (ESCS) in humans. This gene produces a large isoform encoded in 8 exons and a previously unreported shorter isoform of 7 exons, whose function is unknown. We generated two mouse models by targeting exon 8 of Nr2e3 using CRISPR/Cas9-D10A nickase. Allele Δ27 is an in-frame deletion of 27 bp that ablates the dimerization domain H10, whereas allele ΔE8 (full deletion of exon 8) produces only the short isoform, which lacks the C-terminal part of the ligand binding domain (LBD) that encodes both H10 and the AF2 domain involved in the Nr2e3 repressor activity. The Δ27 mutant shows developmental alterations and a non-progressive electrophysiological dysfunction that resembles the ESCS phenotype. The ΔE8 mutant exhibits progressive retinal degeneration, as occurs in human RP patients. Our mutants suggest a role for Nr2e3 as a cone-patterning regulator and provide valuable models for studying mechanisms of NR2E3-associated retinal dystrophies and evaluating potential therapies. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.nbd.2020.105122 |
It is part of: | Neurobiology of Disease, 2020, vol. 146, p. 105122 |
URI: | http://hdl.handle.net/2445/172472 |
Related resource: | https://doi.org/10.1016/j.nbd.2020.105122 |
ISSN: | 0969-9961 |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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