Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/172474
Title: | T-type calcium channels drive migration/invasion in BRAFV600E melanoma cells through Snail1 |
Author: | Maiques, Oscar Barceló, Carla Panosa, Anaïs Pijuan, Jordi Orgaz, Jose L. Rodríguez Hernández, Irene Matas Nadal, Clara Tell Martí, Gemma Vilella, Ramón Fabra Fres, Àngels Puig i Sardà, Susana Sanz Moreno, Victoria Matias-Guiu, Xavier Canti, Carles Herreros, Judit Marti, Rosa M. Macià, Anna |
Keywords: | Melanoma Quimioteràpia Melanoma Chemotherapy |
Issue Date: | 1-Jul-2018 |
Publisher: | Wiley |
Abstract: | Melanoma is a malignant tumor derived from melanocytes. Once disseminated, it is usually highly resistant to chemotherapy and is associated with poor prognosis. We have recently reported that T-type calcium channels (TTCCs) are overexpressed in melanoma cells and play an important role in melanoma progression. Importantly, TTCC pharmacological blockers reduce proliferation and deregulate autophagy leading to apoptosis. Here, we analyze the role of autophagy during migration/invasion of melanoma cells. TTCC Cav3.1 and LC3-II proteins are highly expressed in BRAFV600E compared with NRAS mutant melanomas, both in cell lines and biopsies. Chloroquine, pharmacological blockade, or gene silencing of TTCCs inhibit the autophagic flux and impair the migration and invasion capabilities, specifically in BRAFV600E melanoma cells. Snail1 plays an important role in motility and invasion of melanoma cells. We show that Snail1 is strongly expressed in BRAFV600E melanoma cells and patient biopsies, and its expression decreases when autophagy is blocked. These results demonstrate a role of Snail1 during BRAFV600E melanoma progression and strongly suggest that targeting macroautophagy and, particularly TTCCs, might be a good therapeutic strategy to inhibit metastasis of the most common melanoma type (BRAFV600E). |
Note: | Reproducció del document publicat a: https://doi.org/10.1111/pcmr.12690 |
It is part of: | Pigment Cell & Melanoma Research, 2018, vol. 31, num. 4, p. 484-495 |
URI: | http://hdl.handle.net/2445/172474 |
Related resource: | https://doi.org/10.1111/pcmr.12690 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
MaiquesO.pdf | 1.07 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.