Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/172779
Title: Nicotinamide Prevents apolipoprotein b-containing lipoprotein oxidation, inflammation and atherosclerosis in apolipoprotein e-deficient mice
Author: Méndez-Lara, Karen Alejandra
Letelier Torres, Nicole
Farré, Núria
Diarte-Añazco, Elena M.G.
Nieto-Nicolau, Núria
Rodríguez-Millán, Elisabeth
Santos, David
Pallarès, Víctor
Escolà Gil, Joan Carles
Vázquez Del Olmo, Tania
Lerma, Enrique
Camacho, Mercedes
Casaroli Marano, Ricardo Pedro
Valledor Fernández, Annabel
Blanco-Vaca, Francisco
Julve, Josep
Keywords: Aterosclerosi
Vitamines B
Assaigs clínics de medicaments
Atherosclerosis
Vitamin B complex
Drug testing
Issue Date: 21-Nov-2020
Publisher: MDPI
Abstract: The potential of nicotinamide (NAM) to prevent atherosclerosis has not yet been examined. This study investigated the effect of NAM supplementation on the development of atherosclerosis in a mouse model of the disease. The development of aortic atherosclerosis was significantly reduced (NAM low dose: 45%; NAM high dose: 55%) in NAM-treated, apolipoprotein (Apo)E-deficient mice challenged with a Western diet for 4 weeks. NAM administration significantly increased (1.8-fold) the plasma concentration of proatherogenic ApoB-containing lipoproteins in NAM high-dose (HD)-treated mice compared with untreated mice. However, isolated ApoB-containing lipoproteins from NAM HD mice were less prone to oxidation than those of untreated mice. This result was consistent with the decreased (1.5-fold) concentration of oxidized low-density lipoproteins in this group. Immunohistochemical staining of aortas from NAM-treated mice showed significantly increased levels of IL-10 (NAM low-dose (LD): 1.3-fold; NAM HD: 1.2-fold), concomitant with a significant decrease in the relative expression of TNFα (NAM LD: −44%; NAM HD: −57%). An improved anti-inflammatory pattern was reproduced in macrophages cultured in the presence of NAM. Thus, dietary NAM supplementation in ApoE-deficient mice prevented the development of atherosclerosis and improved protection against ApoB-containing lipoprotein oxidation and aortic inflammation.
Note: Reproducció del document publicat a: https://doi.org/10.3390/antiox9111162
It is part of: Antioxidants, 2020, vol. 9, num. 11, p. 1162
URI: http://hdl.handle.net/2445/172779
Related resource: https://doi.org/10.3390/antiox9111162
ISSN: 2076-3921
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

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