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Title: | Tuning G-quadruplex nanostructures with lipids. Towards designing hybrid scaffolds for oligonucleotide delivery |
Author: | Grijalvo, Santiago Clua, Anna Eres, Marc Gargallo Gómez, Raimundo Eritja i Casadellà, Ramon |
Keywords: | Oligonucleòtids Dicroisme circular Lípids Nanoestructures Oligonucleotides Circular dichroism Lipids Nanostructures |
Issue Date: | 24-Dec-2020 |
Publisher: | MDPI |
Abstract: | Two G-quadruplex forming oligonucleotides [d(TG4T)4 and d(TG6T)4] were selected as two tetramolecular quadruplex nanostructures because of their demonstrated ability to be modified with hydrophobic molecules. This allowed us to synthesize two series of G-quadruplex conjugates that differed in the number of G-tetrads, as well as in the terminal position of the lipid modification. Both solution and solid-phase syntheses were carried out to yield the corresponding lipid oligonucleotide conjugates modified at their 3′- and 5′-termini, respectively. Biophysical studies confirmed that the presence of saturated alkyl chains with different lengths did not affect the G-quadruplex integrity, but increased the stability. Next, the G-quadruplex domain was added to an 18-mer antisense oligonucleotide. Gene silencing studies confirmed the ability of such G-rich oligonucleotides to facilitate the inhibition of target Renilla luciferase without showing signs of toxicity in tumor cell lines. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/ijms22010121 |
It is part of: | International Journal of Molecular Sciences, 2020, vol. 22, p. 121 |
URI: | http://hdl.handle.net/2445/173049 |
Related resource: | https://doi.org/10.3390/ijms22010121 |
ISSN: | 1661-6596 |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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