Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/173093
Title: Two multi-fragment recombination events resulted in the beta-lactam-resistant serotype 11A-ST6521 related to Spain9V-ST156 pneumococcal clone spreading in south-western Europe, 2008 to 2016
Author: González Díaz, Aida
Machado, Miguel P
Cámara, Jordi
Yuste, José
Varon, Emmanuelle
Domenech, Miriam
Grosso, María Del
Marimón, José María
Cercenado, Emilia
Larrosa, Nieves
Quesada, María Dolores
Fontanals, Dionisia
El-Mniai, Assiya
Cubero, Meritxell
Carrico, João A.
Martí Martí, Sara
Ramirez, Mario
Ardanuy Tisaire, María Carmen
Keywords: Infeccions per pneumococs
Vacunes
Pneumococcal infections
Vaccines
Issue Date: 23-Apr-2020
Publisher: European Centre for Disease Prevention and Control
Abstract: Background: The successful pneumococcal clone Spain(9V)-ST156 (PMEN3) is usually associated with vaccine serotypes 9V and 14. Aim: Our objective was to analyse the increase of a serotype 11A variant of PMEN3 as cause of invasive pneumococcal disease (IPD) in Spain and its spread in south-western Europe. Methods: We conducted a prospective multicentre study of adult IPD in Spain (2008-16). Furthermore, a subset of 61 penicillin-resistant serotype 11A isolates from France, Italy, Portugal and Spain were subjected to whole genome sequencing (WGS) and compared with 238 genomes from the European Nucleotide Archive (ENA). Results: Although the incidence of serotype 11A in IPD was stable, a clonal shift was detected from CC62 (penicillin-susceptible) to CC156 (penicillin-resistant). By WGS, three major 11A-CC156 lineages were identified, linked to ST156 (n=5 isolates; France, Italy and Portugal), ST166 (n=4 isolates; France and Portugal) and ST838/6521 (n=52 isolates; France, Portugal and Spain). Acquisition of the capsule allowed to escape vaccine effect. AP200 (11A-ST62) was the donor for ST156 and ST838/6521 but not for ST166. In-depth analysis of ST838/6521 lineage showed two multi-fragment recombination events including four and seven fragments from an 11A-ST62 and an NT-ST344 representative, respectively. Conclusion: The increase in penicillin-resistant serotype 11A IPD in Spain was linked to the spread of a vaccine escape PMEN3 recombinant clone. Several recombination events were observed in PMEN3 acquiring an 11A capsule. The most successful 11A-PMEN3 lineage spreading in south-western Europe appeared after two multi-fragment recombination events with representatives of two major pneumococcal clones (11A-ST62 and NT-ST344).
Note: Reproducció del document publicat a: https://doi.org/10.2807/1560-7917.ES.2020.25.16.1900457
It is part of: Eurosurveillance, 2020, vol. 25, num. 16, p. 42-51
URI: http://hdl.handle.net/2445/173093
Related resource: https://doi.org/10.2807/1560-7917.ES.2020.25.16.1900457
Appears in Collections:Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Patologia i Terapèutica Experimental)

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