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Title: Directed differentiation of human pluripotent stem sells for the generation of high-order kidney organoids
Author: Selfa, Idoia Lucía
Gallo, Maria
Montserrat, Nuria
Garreta, Elena
Keywords: Ronyó
Cèl·lules mare
Diferenciació cel·lular
Stem cells
Cell diferentiation
Issue Date: 20-Dec-2020
Publisher: Springer Nature
Citation: Selfa I.L., Gallo M., Montserrat N., Garreta E. (2021) Directed Differentiation of Human Pluripotent Stem Cells for the Generation of High-Order Kidney Organoids. In: Ebrahimkhani M.R., Hislop J. (eds) Programmed Morphogenesis. Methods in Molecular Biology, vol 2258. Humana, New York, NY.
Abstract: Our understanding in the inherent properties of human pluripotent stem cells (hPSCs) have made possible the development of differentiation procedures to generate three-dimensional tissue-like cultures, so-called organoids. Here we detail a stepwise methodology to generate kidney organoids from hPSCs. This is achieved through direct differentiation of hPSCs in two-dimensional monolayer culture toward the posterior primitive streak fate, followed by induction of intermediate mesoderm-committed cells, which are further aggregated and cultured in three-dimensions to generate kidney organoids containing segmented nephron-like structures in a process that lasts 20 days. We also provide a concise description on how to assess renal commitment during the time course of kidney organoid generation. This includes the use of flow cytometry and immunocytochemistry analyses for the detection of specific renal differentiation markers.
Note: Versió postprint del document publicat a:
It is part of: Capítol del llibre: Mo R. Ebrahimkhani and Joshua Hislop (eds.), Programmed Morphogenesis: Methods and Protocols, Methods in Molecular Biology, vol. 2258,, Springer Nature 2021. Chapter 12
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Appears in Collections:Publicacions de projectes de recerca finançats per la UE
Llibres / Capítols de llibre (Institut de Bioenginyeria de Catalunya (IBEC))

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