Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/173498
Title: | The HERC1 ubiquitin ligase regulates presynaptic membrane dynamics of central synapses |
Author: | Montes Fernández, Mª Angeles Pérez Villegas, Eva María Garcia Gonzalo, Francesc Pedrazza, Leonardo Rosa López, José Luis Alvarez de Toledo, Guillermo Armengol, José Ángel |
Keywords: | Ubiqüitina Autofàgia Malalties neurodegeneratives Ubiquitin Autophagy Neurodegenerative Diseases |
Issue Date: | 21-Jul-2020 |
Publisher: | Nature Publishing Group |
Abstract: | HERC1 is a ubiquitin ligase protein, which, when mutated, induces several malformations and intellectual disability in humans. The animal model of HERC1 mutation is the mouse tambaleante characterized by: (1) overproduction of the protein; (2) cerebellar Purkinje cells death by autophagy; (3) dysregulation of autophagy in spinal cord motor neurons, and CA3 and neocortical pyramidal neurons; (4) impairment of associative learning, linked to altered spinogenesis and absence of LTP in the lateral amygdala; and, (5) motor impairment due to delayed action potential transmission, decrease synaptic transmission efficiency and altered myelination in the peripheral nervous system. To investigate the putative role of HERC1 in the presynaptic dynamics we have performed a series of experiments in cultured tambaleante hippocampal neurons by using transmission electron microscopy, FM1-43 destaining and immunocytochemistry. Our results show: (1) a decrease in the number of synaptic vesicles; (2) reduced active zones; (3) less clathrin immunoreactivity and less presynaptic endings over the hippocampal main dendritic trees; which contrast with (4) a greater number of endosomes and autophagosomes in the presynaptic endings of the tambaleante neurons relative to control ones. Altogether these results show an important role of HERC1 in the regulation of presynaptic membrane dynamics. |
Note: | Reproducció del document publicat a: https://doi.org/10.1038/s41598-020-68970-8 |
It is part of: | Scientific Reports, 2020, vol. 10 |
URI: | http://hdl.handle.net/2445/173498 |
Related resource: | https://doi.org/10.1038/s41598-020-68970-8 |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Montes-FernandezMA.pdf | 29.15 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License