Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/173837
Title: Differential adaptability between reference strains and clinical isolates of Pseudomonas aeruginosa into the lung epithelium intracellular lifestyle
Author: Cendra Gascón, María del Mar
Torrents Serra, Eduard
Keywords: Bacteris patògens
Epiteli
Pathogenic bacteria
Epithelium
Issue Date: 22-Jul-2020
Publisher: Taylor and Francis
Abstract: Intracellular invasion is an advantageous mechanism used by pathogens to evade host defense and antimicrobial therapy. In patients, the intracellular microbial lifestyle can lead to infection persistence and recurrence, thus worsening outcomes. Lung infections caused by Pseudomonas aeruginosa, especially in cystic fibrosis (CF) patients, are often aggravated by intracellular invasion and persistence of the pathogen. Proliferation of the infectious species relies on a continuous deoxyribonucleotide (dNTP) supply, for which the ribonucleotide reductase enzyme (RNR) is the unique provider. The large genome plasticity of P. aeruginosa and its ability to rapidly adapt to different environments are challenges for studying the pathophysiology associated with this type of infection. Using different reference strains and clinical isolates of P. aeruginosa independently combined with alveolar (A549) and bronchial (16HBE14o- and CF-CFBE41o-) epithelial cells, we analyzed host-pathogen interactions and intracellular bacterial persistence with the aim of determining a cell type-directed infection promoted by the P. aeruginosa strains. The oscillations in cellular toxicity and oxygen consumption promoted by the intracellular persistence of the strains were also analyzed among the different infectious lung models. Significantly, we identified class II RNR as the enzyme that supplies dNTPs to intracellular P. aeruginosa. This discovery could contribute to the development of RNR-targeted strategies against the chronicity occurring in this type of lung infection. Overall our study demonstrates that the choice of bacterial strain is critical to properly study the type of infectious process with relevant translational outcomes.
Note: Versió postprint del document publicat a: https://doi.org/10.1080/21505594.2020.1787034
It is part of: Virulence, 2020, vol. 11, num. 1, p. 862-876
URI: http://hdl.handle.net/2445/173837
Related resource: https://doi.org/10.1080/21505594.2020.1787034
ISSN: 2150-5594
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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