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Title: Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue
Author: Mendive Tapia, Lorena
Zhao, Can
Akram, Ahsan R.
Preciado Gallego, Sara
Albericio Palomera, Fernando
Lee, Martin
Serrels, Alan
Kielland, Nicola
Read, Nick D.
Lavilla Grífols, Rodolfo
Vendrell, Marc
Keywords: Pèptids
Aspergillus parasiticus
Aspergillus parasiticus
Amino acids
Issue Date: 9-Mar-2016
Publisher: Nature Publishing Group
Abstract: Fluorescent antimicrobial peptides are promising structures for in situ, real-time imaging of fungal infection. Here we report a fluorogenic probe to image Aspergillus fumigatus directly in human pulmonary tissue. We have developed a fluorogenic Trp-BODIPY amino acid with a spacer-free C-C linkage between Trp and a BODIPY fluorogen, which shows remarkable fluorescence enhancement in hydrophobic microenvironments. The incorporation of our fluorogenic amino acid in short antimicrobial peptides does not impair their selectivity for fungal cells, and enables rapid and direct fungal imaging without any washing steps. We have optimized the stability of our probes in human samples to perform multi-photon imaging of A. fumigatus in ex vivo human tissue. The incorporation of our unique BODIPY fluorogen in biologically relevant peptides will accelerate the development of novel imaging probes with high sensitivity and specificity.
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It is part of: Nature Communications, 2016, vol. 7, p. 10940
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ISSN: 2041-1723
Appears in Collections:Articles publicats en revistes (Química Inorgànica i Orgànica)

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