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|Title:||Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue|
|Author:||Mendive Tapia, Lorena|
Akram, Ahsan R.
Preciado Gallego, Sara
Albericio Palomera, Fernando
Read, Nick D.
Lavilla Grífols, Rodolfo
|Publisher:||Nature Publishing Group|
|Abstract:||Fluorescent antimicrobial peptides are promising structures for in situ, real-time imaging of fungal infection. Here we report a fluorogenic probe to image Aspergillus fumigatus directly in human pulmonary tissue. We have developed a fluorogenic Trp-BODIPY amino acid with a spacer-free C-C linkage between Trp and a BODIPY fluorogen, which shows remarkable fluorescence enhancement in hydrophobic microenvironments. The incorporation of our fluorogenic amino acid in short antimicrobial peptides does not impair their selectivity for fungal cells, and enables rapid and direct fungal imaging without any washing steps. We have optimized the stability of our probes in human samples to perform multi-photon imaging of A. fumigatus in ex vivo human tissue. The incorporation of our unique BODIPY fluorogen in biologically relevant peptides will accelerate the development of novel imaging probes with high sensitivity and specificity.|
|Note:||Reproducció del document publicat a: https://doi.org/10.1038/ncomms10940|
|It is part of:||Nature Communications, 2016, vol. 7, p. 10940|
|Appears in Collections:||Articles publicats en revistes (Química Inorgànica i Orgànica)|
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