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http://hdl.handle.net/2445/174930
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DC Field | Value | Language |
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dc.contributor.author | Kalafatovic, Daniela | - |
dc.contributor.author | Mausa, Goran | - |
dc.contributor.author | Resetar Maslov, Dina | - |
dc.contributor.author | Giralt Lledó, Ernest | - |
dc.date.accessioned | 2021-03-11T12:51:44Z | - |
dc.date.available | 2021-03-11T12:51:44Z | - |
dc.date.issued | 2020-07-22 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | http://hdl.handle.net/2445/174930 | - |
dc.description.abstract | One-bead-one-compound peptide libraries, developed following the top-down experimental approach, have attracted great interest in the identification of potential ligands or active peptides. By exploiting a reverse experimental design approach based on the bottom-up strategy, we aimed to develop simplified, maximally diverse peptide libraries that resulted in the successful characterization of mixture components. We show that libraries of 32 and 48 components can be successfully detected in a single run using chromatography coupled to mass spectrometry (UPLC-MS). The proposed libraries were further theoretically evaluated in terms of their composition and physico-chemical properties. By combining the knowledge obtained on single libraries we can cover larger sequence spaces and provide a controlled exploration of the peptide chemical space both theoretically and experimentally. Designing libraries by using the bottom-up approach opens up the possibility of rationally fine-tuning the library complexity based on the available analytical methods. | - |
dc.format.extent | 15 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | MDPI | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3390/molecules25153316 | - |
dc.relation.ispartof | Molecules, 2020, vol. 25(15), num. 3316 | - |
dc.relation.uri | https://doi.org/10.3390/molecules25153316 | - |
dc.rights | cc-by (c) Kalafatovic, Daniela et al., 2020 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Química Inorgànica i Orgànica) | - |
dc.subject.classification | Síntesi de pèptids | - |
dc.subject.classification | Síntesi en fase sólida | - |
dc.subject.classification | Química combinatòria | - |
dc.subject.other | Peptide synthesis | - |
dc.subject.other | Solid-phase synthesis | - |
dc.subject.other | Combinatorial chemistry | - |
dc.title | Bottom-Up Design Approach for OBOC Peptide Libraries | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 703956 | - |
dc.date.updated | 2021-03-11T12:51:44Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 32707811 | - |
Appears in Collections: | Articles publicats en revistes (Química Inorgànica i Orgànica) Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
Files in This Item:
File | Description | Size | Format | |
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703956.pdf | 2.66 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License