Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/174950
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Fuentes Pardo, Cristina | - |
dc.contributor.author | Pérez-Rodríguez, Francisco Javier | - |
dc.contributor.author | Sabrià Sunyé, Aurora | - |
dc.contributor.author | Beguiristain Celayeta, Nerea | - |
dc.contributor.author | Pintó Solé, Rosa María | - |
dc.contributor.author | Guix Arnau, Susana | - |
dc.contributor.author | Bosch, Albert | - |
dc.date.accessioned | 2021-03-11T14:30:12Z | - |
dc.date.available | 2021-03-11T14:30:12Z | - |
dc.date.issued | 2021-01-12 | - |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | http://hdl.handle.net/2445/174950 | - |
dc.description.abstract | Bivalve mollusk contamination by enteric viruses, especially human noroviruses (HuNoV) and hepatitis A virus (HAV), is a problem with health and economic implications. The aim of the study was the evaluation of the effect of heat treatment in clams (Tawera gayi) experimentally contaminated with HuNoV using a PMA-viability RTqPCR assay to minimize measurement of non-infectious viruses, and used HAV as a model to estimate infectivity loss. Spiked clams were immersed in water at 90°C to ensure that internal meat temperature was maintained above 90°C for at least 5 min. The treatment resulted in >3.89 ± 0.24 log10 TCID50/g reduction of infectious HAV, confirming inactivation. For HuNoV, RTqPCR assays showed log10 reductions of 2.96 ± 0.79 and 2.56 ± 0.56, for GI and GII, respectively, and the use of PMA resulted in an additional log10 reduction for GII, providing a better correlation with risk reduction. In the absence of a cell culture system which could be used to determine HuNoV infectivity reduction, a performance criteria based on PMA-RTqPCR log reduction could be used to evaluate food product safety. According to data from this study, heat treatments of clams which cause reductions >3.5 log10 for GII as measured by PMA-RTqPCR assay may be regarded as an acceptable inactivation treatment, and could be set as a performance criterion to test the effectiveness of other time-temperature inactivation processes. | - |
dc.format.extent | 6 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Frontiers Media | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2020.578328 | - |
dc.relation.ispartof | Frontiers in Microbiology, 2021, vol. 11, p. 578328 | - |
dc.relation.uri | https://doi.org/10.3389/fmicb.2020.578328 | - |
dc.rights | cc-by (c) Fuentes Pardo, Cristina et al., 2021 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Hepatitis A | - |
dc.subject.classification | Terapèutica | - |
dc.subject.other | Hepatitis A | - |
dc.subject.other | Therapeutics | - |
dc.title | Inactivation of hepatitis A virus and human norovirus in clams subjected to heat treatment | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 707144 | - |
dc.date.updated | 2021-03-11T14:30:12Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/727580/EU//SafeConsumE | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 33510715 | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) Publicacions de projectes de recerca finançats per la UE |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
707144.pdf | 176.14 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License