Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/17522| Title: | Development and application of a one-step low cost procedure to concentrate viruses from seawater samples |
| Author: | Calgua de León, Byron Thomas Mengewein, A. Grünert, Andreas Bofill Mas, Silvia Clemente Casares, Pilar Hundesa Gonfa, Ayalkibet Wyn-Jones, A. Peter López-Pila, Juan Gironès Llop, Rosina |
| Keywords: | Adenovirus Aigua de mar Adenoviruses Seawater |
| Issue Date: | 2008 |
| Publisher: | Elsevier B.V. |
| Abstract: | A novel and simple procedure for concentrating adenoviruses from seawater samples is described. The technique entails the adsorption of viruses to pre-flocculated skimmed milk proteins, allowing the flocs to sediment by gravity, and dissolving the separated sediment in phosphate buffer. Concentrated virus may be detected by PCR techniques following nucleic acid extraction. The method requires no specialized equipment other than that usually available in routine public health laboratories, and due to its straightforwardness it allows the processing of a larger number of water samples simultaneously. The usefulness of the method was demonstrated in concentration of virus in multiple seawater samples during a survey of adenoviruses in coastal waters. |
| Note: | Versió postprint del document publicat a http://dx.doi.org/10.1016/j.jviromet.2008.08.003 |
| It is part of: | Journal of Virological Methods, 2008, volumen 153, p. 79-83 |
| URI: | http://hdl.handle.net/2445/17522 |
| Related resource: | http://dx.doi.org/10.1016/j.jviromet.2008.08.003 |
| ISSN: | 0166-0934 |
| Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) Publicacions de projectes de recerca finançats per la UE |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 564764.pdf | 129.9 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.