Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/175288
Title: Targeting hepatic protein carbonylation and oxidative stress occurring on diet-induced metabolic diseases through the supplementation with fish oils
Author: Muñoz, Silvia
Méndez, Lucia
Dasilva, Gabriel
Torres Simón, Josep Lluís
Ramos Romero, Sara
Romeu Ferran, Marta
Nogués, Maria Rosa
Medina, Isabel
Keywords: Olis i greixos comestibles
Trastorns del metabolisme
Peixos
Edible oils and fats
Disorders of metabolism
Fishes
Issue Date: 2018
Publisher: MDPI
Abstract: The present study addressed the ability of long-chain ω-3 polyunsaturated fatty acids (ω-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate liver protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ω-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated proteins involved in specific metabolic pathways. Results showed that the intake of marine ω-3 PUFA through diet significantly decreased liver protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty liver proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ω-3 PUFA. In parallel, fish oil significantly changed the liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ω-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in liver.
Note: Reproducció del document publicat a: https://doi.org/10.3390/md16100353
It is part of: Marine Drugs, 2018, vol. 16, num. 10, p. 353-376
URI: http://hdl.handle.net/2445/175288
Related resource: https://doi.org/10.3390/md16100353
ISSN: 1660-3397
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

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