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Title: | Function of the Pseudomonas aeruginosa NrdR Transcription Factor: Global Transcriptomic Analysis and Its Role on Ribonucleotide Reductase Gene Expression |
Author: | Crespo Puig, Anna Pedraz López, Lucas Torrents Serra, Eduard |
Keywords: | Pseudomonas Expressió gènica ADN Pseudomonas Gene expression DNA |
Issue Date: | 24-Apr-2015 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Ribonucleotide reductases (RNRs) are a family of sophisticated enzymes responsible for the synthesis of the deoxyribonucleotides (dNTPs), the building blocks for DNA synthesis and repair. Although any living cell must contain one RNR activity to continue living, bacteria have the capacity to encode different RNR classes in the same genome, allowing them to adapt to different environments and growing conditions. Pseudomonas aeruginosa is well known for its adaptability and surprisingly encodes all three known RNR classes (Ia, II and III). There must be a complex transcriptional regulation network behind this RNR activity, dictating which RNR class will be expressed according to specific growing conditions. In this work, we aim to uncover the role of the transcriptional regulator NrdR in P. aeruginosa. We demonstrate that NrdR regulates all three RNR classes, being involved in differential control depending on whether the growth conditions are aerobic or anaerobic. Moreover, we also identify for the first time that NrdR is not only involved in controlling RNR expression but also regulates topoisomerase I (topA) transcription. Finally, to obtain the entire picture of NrdR regulon, we performed a global transcriptomic analysis comparing the transcription profile of wild-type and nrdR mutant strains. The results provide many new data about the regulatory network that controls P. aeruginosa RNR transcription, bringing us a step closer to the understanding of this complex system. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0123571 |
It is part of: | PLoS One, 2015, vol. 10, num. 4, p. e0123571 |
URI: | http://hdl.handle.net/2445/175495 |
Related resource: | https://doi.org/10.1371/journal.pone.0123571 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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