Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/175538
Title: | Diagnostic accuracy of five serologic tests for Strongyloides stercoralis infection. |
Author: | Bisoffi, Zeno Buonfrate, Dora Sequi, Marco Mejia, Rojelio Cimino, Rubén O. Krolewiecki, Alejandro J. Albonico, Marco Gobbo, Maria Bonafini, Stefania Angheben, Andrea Requena-Méndez, Ana Muñoz Gutiérrez, José Nutman, Thomas B. |
Keywords: | Malalties parasitàries Larves Serodiagnòstic Parasitic diseases Larvae Serodiagnosis |
Issue Date: | 9-Jan-2014 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Background: The diagnosis of Strongyloides stercoralis (S. stercoralis) infection is hampered by the suboptimal sensitivity of fecal-based tests. Serological methods are believed to be more sensitive, although assessing their accuracy is difficult because of the lack of sensitivity of a fecal-based reference ('gold') standard. Methods: The sensitivity and specificity of 5 serologic tests for S. stercoralis (in-house IFAT, NIE-ELISA and NIE-LIPS and the commercially available Bordier-ELISA and IVD-ELISA) were assessed on 399 cryopreserved serum samples. Accuracy was measured using fecal results as the primary reference standard, but also using a composite reference standard (based on a combination of tests). Results: According to the latter standard, the most sensitive test was IFAT, with 94.6% sensitivity (91.2-96.9), followed by IVD-ELISA (92.3%, 87.7-96.9). The most specific test was NIE-LIPS, with specificity 99.6% (98.9-100), followed by IVD-ELISA (97.4%, 95.5-99.3). NIE-LIPS did not cross-react with any of the specimens from subjects with other parasitic infections. NIE-LIPS and the two commercial ELISAs approach 100% specificity at a cut off level that maintains ≥70% sensitivity. Conclusions: NIE-LIPS is the most accurate serologic test for the diagnosis of S. stercoralis infection. IFAT and each of the ELISA tests are sufficiently accurate, above a given cut off, for diagnosis, prevalence studies and inclusion in clinical trials. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pntd.0002640 |
It is part of: | PLoS Neglected Tropical Diseases, 2014, vol. 8, num. 1, p. e2640 |
URI: | http://hdl.handle.net/2445/175538 |
Related resource: | https://doi.org/10.1371/journal.pntd.0002640 |
ISSN: | 1935-2735 |
Appears in Collections: | Articles publicats en revistes (Medicina) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
704132.pdf | 897.81 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License