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http://hdl.handle.net/2445/175917
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DC Field | Value | Language |
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dc.contributor.author | Chavarria-Miró, Gemma | - |
dc.contributor.author | Anfruns-Estrada, Eduard | - |
dc.contributor.author | Martínez-Velázquez, Adán | - |
dc.contributor.author | Vázquez-Portero, Mario | - |
dc.contributor.author | Guix Arnau, Susana | - |
dc.contributor.author | Paraira, Miquel | - |
dc.contributor.author | Galofré, Belén | - |
dc.contributor.author | Sánchez, Gloria | - |
dc.contributor.author | Pintó Solé, Rosa María | - |
dc.contributor.author | Bosch, Albert | - |
dc.date.accessioned | 2021-03-30T15:29:06Z | - |
dc.date.available | 2021-10-01T05:10:22Z | - |
dc.date.issued | 2021-03 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | http://hdl.handle.net/2445/175917 | - |
dc.description.abstract | Two large wastewater treatment plants (WWTP), covering around 2.7 M inhabitants, which represents around 85% of the metropolitan area of Barcelona, were sampled before, during and after the implementation of a complete lockdown. Five one-step RT-qPCR assays, targeting the polymerase (IP2 and IP4), the envelope E and the nucleoprotein (N1 and N2) genome regions, were employed for SARS-CoV-2 RNA detection in 24-h composite wastewater samples concentrated by polyethylene glycol (PEG) precipitation. SARS-CoV-2 was detected in a sewage sample collected 41 days ahead of the declaration of the first COVID-19 case. The evolution of SARS-CoV-2 genome copies in wastewater evidenced the validity of water-based epidemiology to anticipate COVID-19 outbreaks, to evaluate the impact of control measures and even to estimate the burden of shedders, including presymptomatic, asymptomatic, symptomatic and undiagnosed cases. For this latter objective, a model was applied for the estimation of the total number of shedders, evidencing a high proportion of asymptomatic infected individuals. In this way, an infection prevalence of 2.0-6.5% was figured. On the other hand, a proportion of around 0.12% and 0.09% of the total population was determined to be required for positive detection in the two WWTPs. At the end of the lockdown, SARS-CoV-2 RNA apparently disappeared in the WWTPs but could still be detected in grab samples from four urban sewers. Sewer monitoring allowed for location of specific hot spots of COVID-19, enabling the rapid adoption of appropriate mitigation measures. | - |
dc.format.extent | 9 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Microbiology | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1128/AEM.02750-20 | - |
dc.relation.ispartof | Applied and Environmental Microbiology, 2021, vol. 87, num. 7, p. e02750 | - |
dc.relation.uri | https://doi.org/10.1128/AEM.02750-20 | - |
dc.rights | (c) American Society for Microbiology, 2021 | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | SARS-CoV-2 | - |
dc.subject.classification | COVID-19 | - |
dc.subject.classification | Aigües residuals | - |
dc.subject.classification | Barcelona (Catalunya : Àrea metropolitana) | - |
dc.subject.other | SARS-CoV-2 | - |
dc.subject.other | COVID-19 | - |
dc.subject.other | Sewage | - |
dc.subject.other | Barcelona (Catalonia : Metropolitan area) | - |
dc.title | Time-evolution of SARS-CoV-2 in wastewater during the first pandemic wave of COVID-19 in the metropolitan area of Barcelona | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 706440 | - |
dc.date.updated | 2021-03-30T15:29:06Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
Files in This Item:
File | Description | Size | Format | |
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706440.pdf | 1.4 MB | Adobe PDF | View/Open |
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