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Title: Cell therapy with hiPSC-derived RPE cells and RPCs prevents visual function loss in a rat model of retinal degeneration
Author: Salas, Anna
Duarri, Anna
Fontrodona, Laura
Ramírez, Diana Mora
Badia, Anna
Isla Magrané, Helena
Ferreira de Souza, Barbara
Zapata, Miguel Ángel
Raya Chamorro, Ángel
Veiga, Anna
García Arumí, José
Keywords: Teràpia cel·lular
Malalties de la retina
Ratolins (Animals de laboratori)
Cellular therapy
Retinal diseases
Mice (Laboratory animals)
Issue Date: 1-Mar-2021
Publisher: Elsevier B. V.
Abstract: Photoreceptor loss is the principal cause of blindness in retinal degenerative diseases (RDDs). Whereas some therapies exist for early stages of RDDs, no effective treatment is currently available for later stages, and once photoreceptors are lost, the only option to rescue vision is cell transplantation. With the use of the Royal College of Surgeons (RCS) rat model of retinal degeneration, we sought to determine whether combined transplantation of human-induced pluripotent stem cell (hiPSC)-derived retinal precursor cells (RPCs) and retinal pigment epithelial (RPE) cells was superior to RPE or RPC transplantation alone in preserving retinal from degeneration. hiPSC-derived RPCs and RPE cells expressing (GFP) were transplanted into the subretinal space of rats. In vivo monitoring showed that grafted cells survived 12 weeks in the subretinal space, and rats treated with RPE + RPC therapy exhibited better conservation of the outer nuclear layer (ONL) and visual response than RPE-treated or RPC-treated rats. Transplanted RPE cells integrated in the host RPE layer, whereas RPC mostly remained in the subretinal space, although a limited number of cells integrated in the ONL. In conclusion, the combined transplantation of hiPSC-derived RPE and RPCs is a potentially superior therapeutic approach to protect retina from degeneration in RDDs.
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It is part of: Molecular Therapy - Methods & Clinical Development, 2021, vol. 20, p. 688-702
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Appears in Collections:Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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