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http://hdl.handle.net/2445/176218
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DC Field | Value | Language |
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dc.contributor.author | Vergara Gómez, Andrea | - |
dc.contributor.author | Moreno Morales, Javier | - |
dc.contributor.author | Roca, I | - |
dc.contributor.author | Pitart, Cristina | - |
dc.contributor.author | Kostyanev, Tomislav | - |
dc.contributor.author | Rodríguez Baño, Jesús | - |
dc.contributor.author | Goossens, Herman | - |
dc.contributor.author | Marco, F. | - |
dc.contributor.author | Vila Estapé, Jordi | - |
dc.date.accessioned | 2021-04-12T16:03:08Z | - |
dc.date.available | 2021-06-01T05:10:24Z | - |
dc.date.issued | 2020-06-01 | - |
dc.identifier.issn | 0305-7453 | - |
dc.identifier.uri | http://hdl.handle.net/2445/176218 | - |
dc.description.abstract | Objectives: To evaluate and compare the efficacy of real-time PCR (Xpert Carba-R) and loop-mediated isothermal amplification (Eazyplex® SuperBug CRE) for detecting carbapenemase carriage in Enterobacteriaceae directly from bronchoalveolar lavage (BAL). Methods: Negative BAL samples were spiked with 21 well-characterized carbapenemase-producing Enterobacteriaceae strains to a final concentration of 102-104 cfu/mL. Xpert Carba-R (Cepheid, Sunnyvale, CA, USA), which detects five targets (blaKPC, blaNDM, blaVIM, blaOXA-48 and blaIMP-1), and the Eazyplex® SuperBug CRE system (Amplex-Diagnostics GmbH, Germany), which detects seven genes (blaKPC, blaNDM, blaVIM, blaOXA-48, blaOXA-181, blaCTXM-1 and blaCTXM-9), were evaluated for the detection of these genes directly from BAL samples. Results: Xpert Carba-R showed 100% agreement with carbapenemase characterization by PCR and sequencing for all final bacteria concentrations. Eazyplex® SuperBug CRE showed 100%, 80% and 27% agreement with PCR and sequencing when testing 104, 103 and 102 cfu/mL, respectively. False negative results for Eazyplex® SuperBug CRE matched the highest cycle threshold values for Xpert Carba-R. Hands-on time for both assays was about 15 min, but Eazyplex® SuperBug CRE results were available within 30 min, whereas Xpert Carba-R took around 50 min. Conclusions: We here describe the successful use of two commercial diagnostic tests, Xpert Carba-R and Eazyplex® SuperBug CRE, to detect bacterial carbapenem resistance genes directly in lower respiratory tract samples. Our results could be used as proof-of-concept data for validation of these tests for this indication. | - |
dc.format.extent | 5 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Oxford University Press | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1093/jac/dkaa031 | - |
dc.relation.ispartof | Journal of Antimicrobial Chemotherapy, 2020, vol. 75, num. 6, p. 1453-1457 | - |
dc.relation.uri | https://doi.org/10.1093/jac/dkaa031 | - |
dc.rights | (c) Vergara, A. et al., 2020 | - |
dc.source | Articles publicats en revistes (Fonaments Clínics) | - |
dc.subject.classification | Diagnòstic microbiològic | - |
dc.subject.classification | Aparell respiratori | - |
dc.subject.classification | Bacteris | - |
dc.subject.other | Diagnostic microbiology | - |
dc.subject.other | Respiratory organs | - |
dc.subject.other | Bacteria | - |
dc.title | A comparative study between real-time PCR and loop-mediated isothermal amplification to detect carbapenemase and/or ESBL genes in Enterobacteriaceae directly from bronchoalveolar lavage fluid samples | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 707110 | - |
dc.date.updated | 2021-04-12T16:03:08Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 32073602 | - |
Appears in Collections: | Articles publicats en revistes (Fonaments Clínics) Articles publicats en revistes (ISGlobal) |
Files in This Item:
File | Description | Size | Format | |
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707110.pdf | 1.08 MB | Adobe PDF | View/Open |
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