Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/176471
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Muñoz, Alvaro | - |
dc.contributor.author | Romero Moya, Damià | - |
dc.contributor.author | Prieto, Cristina | - |
dc.contributor.author | Ramos-Mejía, Verónica | - |
dc.contributor.author | Agraz-Doblas, Antonio | - |
dc.contributor.author | Varela, Ignacio | - |
dc.contributor.author | Buschbeck, Marcus | - |
dc.contributor.author | Palau de Miguel, Anna | - |
dc.contributor.author | Carvajal-Vergara, Xonia | - |
dc.contributor.author | Giorgetti, Alessandra | - |
dc.contributor.author | Ford, Anthony | - |
dc.contributor.author | Lako, Majlinda | - |
dc.contributor.author | Granada, Isabel | - |
dc.contributor.author | Ruiz-Xivillé, Neus | - |
dc.contributor.author | Rodríguez-Perales, Sandra | - |
dc.contributor.author | Torres-Ruíz, Raul | - |
dc.contributor.author | Stam, Ronald W. | - |
dc.contributor.author | Fuster, Jose Luis | - |
dc.contributor.author | Fraga, Mario F. | - |
dc.contributor.author | Nakanishi, Mahito | - |
dc.contributor.author | Cazzaniga, Gianni | - |
dc.contributor.author | Bardini, Michela | - |
dc.contributor.author | Cobo, Isabel | - |
dc.contributor.author | Bayón, Gustavo F. | - |
dc.contributor.author | Fernández, Agustín F. | - |
dc.contributor.author | Bueno, Clara | - |
dc.contributor.author | Menéndez Buján, Pablo | - |
dc.date.accessioned | 2021-04-19T15:10:46Z | - |
dc.date.available | 2021-04-19T15:10:46Z | - |
dc.date.issued | 2016-10-11 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | http://hdl.handle.net/2445/176471 | - |
dc.description.abstract | Induced pluripotent stem cells (iPSCs) are a powerful tool for disease modeling. They are routinely generated from healthy donors and patients from multiple cell types at different developmental stages. However, reprogramming leukemias is an extremely inefficient process. Few studies generated iPSCs from primary chronic myeloid leukemias, but iPSC generation from acute myeloid or lymphoid leukemias (ALL) has not been achieved. We attempted to generate iPSCs from different subtypes of B-ALL to address the developmental impact of leukemic fusion genes. OKSM(L)-expressing mono/polycistronic-, retroviral/lentiviral/episomal-, and Sendai virus vector-based reprogramming strategies failed to render iPSCs in vitro and in vivo. Addition of transcriptomic-epigenetic reprogramming 'boosters' also failed to generate iPSCs from B cell blasts and B-ALL lines, and when iPSCs emerged they lacked leukemic fusion genes, demonstrating non-leukemic myeloid origin. Conversely, MLL-AF4-overexpressing hematopoietic stem cells/B progenitors were successfully reprogrammed, indicating that B cell origin and leukemic fusion gene were not reprogramming barriers. Global transcriptome/DNA methylome profiling suggested a developmental/differentiation refractoriness of MLL-rearranged B-ALL to reprogramming into pluripotency. | - |
dc.format.extent | 17 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1016/j.stemcr.2016.08.013 | - |
dc.relation.ispartof | Stem Cell Reports, 2016, vol. 7, num. 4, p. 602-618 | - |
dc.relation.uri | https://doi.org/10.1016/j.stemcr.2016.08.013 | - |
dc.rights | cc-by (c) Muñoz, Alvaro et al., 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Leucèmia | - |
dc.subject.classification | Cèl·lules | - |
dc.subject.classification | Genètica | - |
dc.subject.other | Leukemia | - |
dc.subject.other | Cells | - |
dc.subject.other | Genetics | - |
dc.title | Developmental refractoriness of MLL-rearranged human B-cell acute leukemias to reprogramming into pluripotency | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 711118 | - |
dc.date.updated | 2021-04-19T15:10:47Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/646903/EU//INFANTLEUKEMIA | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/266608/EU//E-RARE-2 | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27666791 | - |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Biomedicina) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
711118.pdf | 3.5 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License