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Title: Homing of peripherally injected bone marrow cells in rat after experimental myocardial injury
Author: Ciulla, Michelem
Lazzari, Lorenza
Pacchiana, Raffaella
Esposito, Arturo
Bosari, Silvano
Ferrero, Stefano
Gianelli, Umberto
Paliotti, Roberta
Busca, Giuseppe
Giorgetti, Alessandra
Magrini, Fabio
Rebulla, Paolo
Keywords: Medul·la òssia
Infart de miocardi
Bone marrow
Myocardial infarction
Issue Date: 1-Jun-2003
Publisher: Ferrata Storti Foundation
Abstract: Background and objectives: significant progress has been achieved during the past 10 years in cell transplantation and recent research has focused on the possibility of improving ventricular function after myocardial infarction. Most studies in the field of cardiac tissue repair are performed by direct intramyocardial injection of cells of different origin. Since this approach requires a surgical intervention, in this study we investigated the feasibility of non-invasive administration of bone marrow mononuclear cells (BMMNCs) by assessing the fate of peripherally injected, purified, labeled cells in cryodamaged hearts. Design and methods: ten donor and ten recipient inbred isogenic adult (4 weeks old) Fisher rats were used as models to mimic autologous transplantation. Myocardial damage was obtained in recipient rats by placing a frozen metal probe on the anterior left ventricular wall for 15 seconds (freeze-thaw injury technique). BMMNCs were purified and labeled with a red fluorescent cell dye. Seven days after the injury about 15-25x10(6) cells were infused through the femoral vein of recipient rats. Seven days after the infusion, the heart, lungs, liver, kidneys, spleen and thymus were harvested to track transplanted cells. RESULTS: Labeled cells were found only in the injured area of the heart and not in the normal tissue, and a limited number of cells were identified in the spleen of all the animals. Most of the labeled cells in the infarcted area were Thy-1(+) and some were CD34(+). Interpretation and conclusions: our data suggest that peripherally injected BMMNCs can traffic through the circulation to the site of damage; we hypothesize that tissue injury leads to the priming of a cytokine cascade acting as chemoattractant for the infused cells.
Note: Reproducció del document publicat a:
It is part of: Haematologica, 2003, vol. 88, num. 6, p. 614-621
ISSN: 0390-6078
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)

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