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http://hdl.handle.net/2445/176628
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DC Field | Value | Language |
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dc.contributor.author | Moreno Morales, Javier | - |
dc.contributor.author | Vergara Gómez, Andrea | - |
dc.contributor.author | Kostyanev, Tomislav | - |
dc.contributor.author | Rodríguez Baño, Jesús | - |
dc.contributor.author | Goossens, Herman | - |
dc.contributor.author | Vila Estapé, Jordi | - |
dc.date.accessioned | 2021-04-22T10:36:22Z | - |
dc.date.available | 2021-04-22T10:36:22Z | - |
dc.date.issued | 2021-01-13 | - |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | http://hdl.handle.net/2445/176628 | - |
dc.description.abstract | Carbapenem-resistant Acinetobacter spp. mainly Acinetobacter baumannii are frequently causing nosocomial infections with high mortality. In this study, the efficacy of the Eazyplex® SuperBug Complete A system, based on loop-mediated isothermal amplification (LAMP), to detect the presence of carbapenemases in Acinetobacter spp. directly from bronchoalveolar lavage (BAL) samples was assessed. A total of 22 Acinetobacter spp. strains producing OXA-23, OXA-40, OXA-58, NDM, and IMP were selected. Eazyplex SuperBug Complete A kit, used with the Genie II device, is a molecular diagnostics kit that detects a selection of genes that express carbapenemases (bla KPC , bla NDM , bla VIM , bla OXA-48 , bla OXA-23 , bla OXA-40 , and bla OXA-58 ). Negative BAL samples were identified, McFarland solutions were prepared from each of the 22 Acinetobacter strains and serial dilutions in saline solution were made to finally spike BAL samples to a concentration of 102 and 103 CFU/ml. Fifteen concentrations out of the 44 tested out did not provide detection of the carbapenemase-producing gene, all but one being at the lowest concentration tested at 102 CFU/ml; therefore, the limit of sensitivity is 103 CFU/ml. This assay represents the kind of advantages that investing in molecular diagnostics brings to the clinical practice, allowing the identification of carbapenemases in less than 30 min with a sensitivity of 103 CFU/ml. | - |
dc.format.extent | 4 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Frontiers Media | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2020.597684 | - |
dc.relation.ispartof | Frontiers in Microbiology, 2021, vol. 11, num. 597684 | - |
dc.relation.uri | https://doi.org/10.3389/fmicb.2020.597684 | - |
dc.rights | cc-by (c) Moreno Morales, Javier et al., 2021 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Fonaments Clínics) | - |
dc.subject.classification | Infeccions nosocomials | - |
dc.subject.classification | Enterobacteriàcies | - |
dc.subject.other | Nosocomial infections | - |
dc.subject.other | Enterobacteriaceae | - |
dc.title | Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly from Bronchoalveolar Lavage Fluid Spiked with Acinetobacter spp | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 710656 | - |
dc.date.updated | 2021-04-22T10:36:22Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 33519735 | - |
Appears in Collections: | Articles publicats en revistes (ISGlobal) Articles publicats en revistes (Fonaments Clínics) |
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File | Description | Size | Format | |
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710656.pdf | 188.72 kB | Adobe PDF | View/Open |
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