Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/176686
Title: MED15 prion-like domain forms a coiled-coil responsible for its amyloid conversion and propagation
Author: Batlle, Cristina
Calvo, Isabel
Iglesias, Valentin
Lynch, Cian J.
Gil García, Marcos
Serrano Marugán, Manuel
Ventura, Salvador
Keywords: Prions
Amiloïdosi
Prions
Amyloidosis
Issue Date: 1-Dec-2021
Abstract: A disordered to β-sheet transition was thought to drive the functional switch of Q/N-rich prions, similar to pathogenic amyloids. However, recent evidence indicates a critical role for coiled-coil (CC) regions within yeast prion domains in amyloid formation. We show that many human prion-like domains (PrLDs) contain CC regions that overlap with polyQ tracts. Most of the proteins bearing these domains are transcriptional coactivators, including the Mediator complex subunit 15 (MED15) involved in bridging enhancers and promoters. We demonstrate that the human MED15-PrLD forms homodimers in solution sustained by CC interactions and that it is this CC fold that mediates the transition towards a β-sheet amyloid state, its chemical or genetic disruption abolishing aggregation. As in functional yeast prions, a GFP globular domain adjacent to MED15-PrLD retains its structural integrity in the amyloid state. Expression of MED15-PrLD in human cells promotes the formation of cytoplasmic and perinuclear inclusions, kidnapping endogenous full-length MED15 to these aggregates in a prion-like manner. The prion-like properties of MED15 are conserved, suggesting novel mechanisms for the function and malfunction of this transcription coactivator.
Note: Reproducció del document publicat a: https://doi.org/10.1038/s42003-021-01930-8
It is part of: Communications Biology, 2021, vol. 4
URI: http://hdl.handle.net/2445/176686
Related resource: https://doi.org/10.1038/s42003-021-01930-8
Appears in Collections:Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

Files in This Item:
File Description SizeFormat 
12541_6492001_batlle_et_al_commbiol_.pdf2.93 MBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons