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http://hdl.handle.net/2445/176737
Title: | NCS-1 Inhibits insulin stimulated GLUT4 translocation in 3T3L1 adipocytes through a phosphatidylinositol 4-kinase dependent pathway |
Author: | Mora Fayos, Sílvia Durham, Paul L. Smith, Jeffery R. Russo, Andrew F. Jeromin, Andrea Pessin, Jeffrey E. |
Keywords: | Fosforilació Teixit adipós Receptors d'insulina Phosphorylation Adipose tissues Insulin receptors |
Issue Date: | 26-Jul-2002 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Abstract: | Expression of NCS-1 (neuronal calcium sensor-1, also termed frequenin) in 3T3L1 adipocytes strongly inhibited insulin-stimulated translocation of GLUT4 and insulin-responsive aminopeptidase. The effect of NCS-1 was specific for GLUT4 and the insulin-responsive aminopeptidase translocation as there was no effect on the trafficking of the cation-independent mannose 6-phosphate receptor or the GLUT1 glucose transporter isoform. Moreover, NCS-1 showed partial colocalization with GLUT4-EGFP in the perinuclear region. The inhibitory action of NCS-1 was independent of calcium sequestration since neither treatment with ionomycin nor endothelin-1, both of which elevated the intracellular calcium concentration, restored insulin-stimulated GLUT4 translocation. Furthermore, NCS-1 did not alter the insulin-stimulated protein kinase B (PKB/Akt) phosphorylation or the recruitment of Cbl to the plasma membrane. In contrast, expression of the NCS-1 effector phosphatidylinositol 4-kinase (PI 4-kinase) inhibited insulin-stimulated GLUT4 translocation, whereas co-transfection with an inactive PI 4-kinase mutant prevented the NCS-1-induced inhibition. These data demonstrate that PI 4-kinase functions to negatively regulate GLUT4 translocation through its interaction with NCS-1. |
Note: | Reproducció del document publicat a: https://doi.org/10.1074/jbc.M203669200 |
It is part of: | Journal of Biological Chemistry, 2002, vol. 277, p. 27494-27500 |
URI: | http://hdl.handle.net/2445/176737 |
Related resource: | https://doi.org/10.1074/jbc.M203669200 |
ISSN: | 0021-9258 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
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