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DC Field | Value | Language |
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dc.contributor.author | Kanzaki, Makoto | - |
dc.contributor.author | Mora Fayos, Sílvia | - |
dc.contributor.author | Hwang, Joseph B. | - |
dc.contributor.author | Saltiel, Alan R. | - |
dc.contributor.author | Pessin, Jeffrey E. | - |
dc.date.accessioned | 2021-04-26T16:41:57Z | - |
dc.date.available | 2021-04-26T16:41:57Z | - |
dc.date.issued | 2004-01-20 | - |
dc.identifier.issn | 0021-9525 | - |
dc.identifier.uri | http://hdl.handle.net/2445/176748 | - |
dc.description.abstract | Insulin stimulation of adipocytes resulted in the recruitment of atypical PKC (PKCζ/λ) to plasma membrane lipid raft microdomains. This redistribution of PKCζ/λ was prevented by Clostridium difficile toxin B and by cholesterol depletion, but was unaffected by inhibition of phosphatidylinositol (PI) 3-kinase activity. Expression of the constitutively active GTP-bound form of TC10 (TC10Q/75L), but not the inactive GDP-bound mutant (TC10/T31N), targeted PKCζ/λ to the plasma membrane through an indirect association with the Par6-Par3 protein complex. In parallel, insulin stimulation as well as TC10/Q75L resulted in the activation loop phosphorylation of PKCζ. Although PI 3-kinase activation also resulted in PKCζ/λ phosphorylation, it was not recruited to the plasma membrane. Furthermore, insulin-induced GSK-3β phosphorylation was mediated by both PI 3-kinase-PKB and the TC10-Par6-atypical PKC signaling pathways. Together, these data demonstrate that PKCζ/λ can serve as a convergent downstream target for both the PI 3-kinase and TC10 signaling pathways, but only the TC10 pathway induces a spatially restricted targeting to the plasma membrane. | - |
dc.format.extent | 12 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Rockefeller University Press | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1083/jcb.200306152 | - |
dc.relation.ispartof | Journal of Cell Biology, 2004, vol. 164, p. 279-290 | - |
dc.relation.uri | https://doi.org/10.1083/jcb.200306152 | - |
dc.rights | cc-by (c) Kanzaki, Makoto et al., 2004 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) | - |
dc.subject.classification | Insulina | - |
dc.subject.classification | Fosforilació | - |
dc.subject.other | Insulin | - |
dc.subject.other | Phosphorylation | - |
dc.title | Atypical Protein Kinase C (PKCλ/ζ) is a convergent downstream target of the insulin stimulated phosphatidylinositol 3-kinase and TC10 signalling pathways | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 711522 | - |
dc.date.updated | 2021-04-26T16:41:57Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 14734537 | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
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711522.pdf | 877.2 kB | Adobe PDF | View/Open |
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