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http://hdl.handle.net/2445/176890
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DC Field | Value | Language |
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dc.contributor.author | Wright, David C. | - |
dc.contributor.author | Han, Dong-Ho | - |
dc.contributor.author | García-Roves, Pablo M. (Pablo Miguel) | - |
dc.contributor.author | Geiger, Paige C. | - |
dc.contributor.author | Jones, Terry E. | - |
dc.contributor.author | Holloszy, John O. | - |
dc.date.accessioned | 2021-04-29T10:23:12Z | - |
dc.date.available | 2021-04-29T10:23:12Z | - |
dc.date.issued | 2007-01-05 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/2445/176890 | - |
dc.description.abstract | Exercise results in rapid increases in expression of the transcription coactivator peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha) and in mitochondrial biogenesis in skeletal muscle. PGC-1alpha regulates and coordinates mitochondrial biogenesis, and overexpression of PGC-1alpha in muscle cells results in increases in mitochondrial content. In this context, it has been proposed that the increase in PGC-1alpha protein expression mediates the exercise-induced increase in mitochondrial biogenesis. However, we found that mitochondrial proteins with a short half-life increase as rapidly as, or more rapidly than, PGC-1alpha protein. This finding led us to hypothesize that activation, rather than increased expression, of PGC-1alpha mediates the initial phase of the exercise-induced increase in mitochondria. In this study, we found that most of the PGC-1alpha in resting skeletal muscle is in the cytosol. Exercise resulted in activation of p38 MAPK and movement of PGC-1alpha into the nucleus. In support of our hypothesis, binding of the transcription factor nuclear respiratory factor 1 (NRF-1) to the cytochrome c promoter and NRF-2 to the cytochrome oxidase subunit 4 promoter increased in response to exercise prior to an increase in PGC-1alpha protein. Furthermore, exercise-induced increases in the mRNAs of cytochrome c, delta-aminolevulinate synthase, and citrate synthase also occurred before an increase in PGC-1 protein. Thus, it appears that activation of PGC-1alpha may mediate the initial phase of the exercise-induced adaptive increase in muscle mitochondria, whereas the subsequent increase in PGC-1alpha protein sustains and enhances the increase in mitochondrial biogenesis. | - |
dc.format.extent | 6 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1074/jbc.M606116200 | - |
dc.relation.ispartof | Journal of Biological Chemistry, 2007, vol. 282, num. 1, p. 194-199 | - |
dc.relation.uri | https://doi.org/10.1074/jbc.M606116200 | - |
dc.rights | (c) American Society for Biochemistry and Molecular Biology, 2007 | - |
dc.source | Articles publicats en revistes (Ciències Fisiològiques) | - |
dc.subject.classification | Músculs | - |
dc.subject.classification | Metabolisme | - |
dc.subject.classification | RNA | - |
dc.subject.classification | Factors de transcripció | - |
dc.subject.classification | Proteïnes | - |
dc.subject.other | Muscles | - |
dc.subject.other | Metabolism | - |
dc.subject.other | RNA | - |
dc.subject.other | Transcription factors | - |
dc.subject.other | Proteins | - |
dc.title | Exercise-induced mitochondrial biogenesis begins before the increase in muscle PGC-1alpha expression | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 653063 | - |
dc.date.updated | 2021-04-29T10:23:12Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 17099248 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) |
Files in This Item:
File | Description | Size | Format | |
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653063.pdf | 437.78 kB | Adobe PDF | View/Open |
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