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Title: Chitinase 3-like 1 is neurotoxic in primary cultured neurons
Author: Matute Blanch, Clara
Calvo Barreiro, Laura
Carballo Carbajal, Iria
Gonzálo, Ricardo
Sànchez, Àlex (Sànchez Pla)
Vila, Miquel
Montalban, Xavier
Comabella, Manuel
Keywords: Quitina
Marcadors bioquímics
Esclerosi múltiple
Biochemical markers
Multiple sclerosis
Issue Date: 28-Apr-2020
Publisher: Nature Publishing Group
Abstract: Chitinase 3-like 1 (CHI3L1) is known to play a role as prognostic biomarker in the early stages of multiple sclerosis (MS), and patients with high cerebrospinal fluid CHI3L1 levels have an increased risk for the development of neurological disability. Here, we investigated its potential neurotoxic effect by adding recombinant CHI3L1 in vitro to primary cultures of mouse cortical neurons and evaluating both neuronal functionality and survival by immunofluorescence. CHI3L1 induced a significant neurite length retraction after 24 and 48 hours of exposure and significantly reduced neuronal survival at 48 hours. The cytotoxic effect of CHI3L1 was neuron-specific and was not observed in mouse immune or other central nervous system cells. These results point to a selective neurotoxic effect of CHI3L1 in vitro and suggest a potential role of CHI3L1 as therapeutic target in MS patients.
Note: Reproducció del document publicat a:
It is part of: Scientific Reports, 2020, vol. 10, num. 1, p. 7118
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ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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