Please use this identifier to cite or link to this item:
Title: Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays
Author: Solé Ferré, Anna
Mencía Trinchant, Núria
Villalobos Alberú, Xenia
Noé Mata, Verónica
Ciudad i Gómez, Carlos Julián
Keywords: Micro RNAs
Dianes farmacològiques
Expressió gènica
Drug targeting
Gene expression
Issue Date: 12-Oct-2013
Publisher: BioMed Central
Abstract: BACKGROUND: MicroRNAs are small non-coding RNAs involved in gene expression regulation by targeting specific regions in the 3[prime]-UTR of the mRNA of their target genes. This binding leads to a decrease in the protein levels of such genes either by mRNA degradation or mRNA destabilization and translation inhibition. The interaction between a miRNA and its target mRNAs is usually studied by co-transfection of a reporter expression vector containing the 3[prime]-UTR region of the mRNA and an inhibitory or precursor molecule for the miRNA. This approach, however, does not measure the direct and physical interaction between a miRNA and a specific mRNA. FINDINGS: RNA molecules corresponding to miR-224 and to the 3[prime]-UTR of SLC4A4 were incubated together and their interaction studied under different binding conditions using electrophoretic mobility shift assays. A direct and specific interaction between miR-224 and SLC4A4 mRNA was observed. This interaction was abolished in the presence of competitors. CONCLUSIONS: In this study, we explored a new application for the electrophoretic mobility shift assay and we demonstrated that it is a useful alternative method to assess, in a direct and specific manner, whether a miRNA binds to a specific predicted target mRNA.
Note: Reproducció del document publicat a:
It is part of: BMC Research Notes, 2013, vol. 6, num. 1, p. 454
Related resource:
ISSN: 1756-0500
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
Articles publicats en revistes (Bioquímica i Fisiologia)

Files in This Item:
File Description SizeFormat 
631300.pdf1.68 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons