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DC Field | Value | Language |
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dc.contributor.author | Solé Ferré, Anna | - |
dc.contributor.author | Mencía Trinchant, Núria | - |
dc.contributor.author | Villalobos Alberú, Xenia | - |
dc.contributor.author | Noé Mata, Verónica | - |
dc.contributor.author | Ciudad i Gómez, Carlos Julián | - |
dc.date.accessioned | 2021-05-06T12:10:06Z | - |
dc.date.available | 2021-05-06T12:10:06Z | - |
dc.date.issued | 2013-10-12 | - |
dc.identifier.issn | 1756-0500 | - |
dc.identifier.uri | http://hdl.handle.net/2445/177048 | - |
dc.description.abstract | BACKGROUND: MicroRNAs are small non-coding RNAs involved in gene expression regulation by targeting specific regions in the 3[prime]-UTR of the mRNA of their target genes. This binding leads to a decrease in the protein levels of such genes either by mRNA degradation or mRNA destabilization and translation inhibition. The interaction between a miRNA and its target mRNAs is usually studied by co-transfection of a reporter expression vector containing the 3[prime]-UTR region of the mRNA and an inhibitory or precursor molecule for the miRNA. This approach, however, does not measure the direct and physical interaction between a miRNA and a specific mRNA. FINDINGS: RNA molecules corresponding to miR-224 and to the 3[prime]-UTR of SLC4A4 were incubated together and their interaction studied under different binding conditions using electrophoretic mobility shift assays. A direct and specific interaction between miR-224 and SLC4A4 mRNA was observed. This interaction was abolished in the presence of competitors. CONCLUSIONS: In this study, we explored a new application for the electrophoretic mobility shift assay and we demonstrated that it is a useful alternative method to assess, in a direct and specific manner, whether a miRNA binds to a specific predicted target mRNA. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1186/1756-0500-6-454 | - |
dc.relation.ispartof | BMC Research Notes, 2013, vol. 6, num. 1, p. 454 | - |
dc.relation.uri | https://doi.org/10.1186/1756-0500-6-454 | - |
dc.rights | cc-by (c) Solé Ferré, Anna et al., 2013 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Bioquímica i Fisiologia) | - |
dc.subject.classification | Micro RNAs | - |
dc.subject.classification | RNA | - |
dc.subject.classification | Dianes farmacològiques | - |
dc.subject.classification | Expressió gènica | - |
dc.subject.other | MicroRNAs | - |
dc.subject.other | RNA | - |
dc.subject.other | Drug targeting | - |
dc.subject.other | Gene expression | - |
dc.title | Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 631300 | - |
dc.date.updated | 2021-05-06T12:10:06Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 24215842 | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) Articles publicats en revistes (Bioquímica i Fisiologia) |
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File | Description | Size | Format | |
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631300.pdf | 1.68 MB | Adobe PDF | View/Open |
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