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http://hdl.handle.net/2445/177587
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DC Field | Value | Language |
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dc.contributor.author | Aguado Tomàs, Fernando | - |
dc.contributor.author | Gombau, Lourdes | - |
dc.contributor.author | Majó, Glòria | - |
dc.contributor.author | Marsal Tebé, Jordi | - |
dc.contributor.author | Blanco Fernández, Jerónimo | - |
dc.contributor.author | Blasi Cabús, Joan | - |
dc.date.accessioned | 2021-05-25T16:43:05Z | - |
dc.date.available | 2021-05-25T16:43:05Z | - |
dc.date.issued | 1997-10-10 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/2445/177587 | - |
dc.description.abstract | Botulinum neurotoxin type A (BoNT/A) inhibits neurotransmitter release by specific cleavage of SNAP-25, a synaptosome-associated protein also expressed in the ACTH secretory cell line AtT-20. Expression of light chain BoNT/A (L-BoNT/A) gene transfected into AtT-20 cells resulted in a cleaved form of SNAP-25 indistinguishable from that generated by bona fide BoNT/A. L-BoNT/A-transfected cells showed no difference in replication rate, viability, or phenotype, compared with control AtT-20 cells. In contrast, L-BoNT/A-transfected cells could not be induced to secrete ACTH upon stimulation by 8-bromo-cAMP or KCl. In addition, alpha-latrotoxin induced ACTH release from control cells, but not from L-BoNT/A-transfected cells. These experiments suggest an important role for SNAP-25 in regulated secretion from AtT-20 cells and underline the usefulness of this cell system as a tool for the study of the molecular mechanism of peptide hormone secretion. | - |
dc.format.extent | 4 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1074/jbc.272.41.26005 | - |
dc.relation.ispartof | Journal of Biological Chemistry, 1997, vol. 272, num. 41, p. 26005-26008 | - |
dc.relation.uri | https://doi.org/10.1074/jbc.272.41.26005 | - |
dc.rights | (c) American Society for Biochemistry and Molecular Biology, 1997 | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Toxina botulínica | - |
dc.subject.classification | Proteïnes de membrana | - |
dc.subject.classification | Teixit nerviós | - |
dc.subject.classification | Hormones pituïtàries | - |
dc.subject.other | Botulinum toxin | - |
dc.subject.other | Membrane proteins | - |
dc.subject.other | Nerve tissue | - |
dc.subject.other | Pituitary hormones | - |
dc.title | Regulated secretion is impaired in AtT-20 endocrine cells stably transfected with botulinum neurotoxin type A light chain | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 130366 | - |
dc.date.updated | 2021-05-25T16:43:05Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 9325336 | - |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
Files in This Item:
File | Description | Size | Format | |
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130366.pdf | 321.84 kB | Adobe PDF | View/Open |
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