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Title: | Regulation of nucleoside transport regulation by lipopolysaccharide, phorbol esters and TNF in human B lymphocytes |
Author: | Soler Prat, Concepció Felipe Campo, Antonio Mata, João F. Casado, Javier (Casado Merediz) Celada Cotarelo, Antonio Pastor Anglada, Marçal |
Keywords: | Limfòcits Nucleòsids Tumors Farmacologia Lymphocytes Nucleosides Tumors Pharmacology |
Issue Date: | 9-Oct-1998 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Abstract: | Nucleoside transport systems and their regulation in human B-lymphocytes have been characterized using the cell lines Raji and Bare lymphoma syndrome-1 (BLS-1) as experimental models. These cells express at least three different nucleoside transport systems as follows: a nitrobenzylthioinosine-sensitive equilibrative transport system of the es-type, which appears to be associated with hENT1 expression, and two Na+-dependent transport systems that may correspond to N1 and to the recently characterized N5-type, which is nitrobenzylthioinosine-sensitive and guanosine-preferring. B cell activators such as phorbol 12-myristate 13-acetate and lipopolysaccharide (LPS) up-regulate both concentrative transport systems but down-regulate the equilibrative es-type transporter, which correlates with lower hENT1 mRNA levels. These effects are dependent on protein kinase C activity. Phorbol 12-myristate 13-acetate and LPS also induce an increase in tumor necrosis factor-alpha (TNF-alpha) mRNA levels, which suggest that this cytokine may mediate some of the effects triggered by these agents, since addition of TNF-alpha alone can increase N1 and N5 transport activities by a mechanism that also depends on protein kinase C activation. Interestingly, TNF-alpha down-regulates es activity, but this effect cannot be abolished by inhibiting protein kinase C. This study reveals differential regulation of nucleoside transport systems following activation of human B-lymphocyte cell lines by agents of physiological relevance such as TNF-alpha and LPS. Moreover, it indicates that the recently characterized N5 transport system can also be regulated following B cell activation, which may be relevant to lymphocyte physiology and to the treatment of lymphocyte malignancies. |
Note: | Reproducció del document publicat a: https://doi.org/10.1074/jbc.273.41.26939 |
It is part of: | Journal of Biological Chemistry, 1998, vol. 273, num. 41, p. 26939-26945 |
URI: | http://hdl.handle.net/2445/177612 |
Related resource: | https://doi.org/10.1074/jbc.273.41.26939 |
ISSN: | 0021-9258 |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Bioquímica i Biomedicina Molecular) Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
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