Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/177771
Title: A novel long non-coding RNA from NBL2 pericentromeric macrosatellite forms a perinucleolar aggregate structure in colon cancer
Author: Dumbovic, Gabrijela
Biayna, Josep
Banus, Jordi
Samuelsson, Johanna
Roth, Anna
Diederichs, Sven
Alonso, Sergio
Buschbeck, Marcus
Perucho, Manuel
Forcales Fernàndez, Sonia-Vanina
Keywords: Càncer colorectal
Genètica
ADN
Colorectal cancer
Genetics
DNA
Issue Date: 20-Jun-2018
Publisher: Oxford University Press
Abstract: Primate-specific NBL2 macrosatellite is hypomethylated in several types of tumors, yet the consequences of this DNA hypomethylation remain unknown. We show that NBL2 conserved repeats are close to the centromeres of most acrocentric chromosomes. NBL2 associates with the perinucleolar region and undergoes severe demethylation in a subset of colorectal cancer (CRC). Upon DNA hypomethylation and histone acetylation, NBL2 repeats are transcribed in tumor cell lines and primary CRCs. NBL2 monomers exhibit promoter activity, and are contained within novel, non-polyA antisense lncRNAs, which we designated TNBL (Tumor-associated NBL2 transcript). TNBL is stable throughout the mitotic cycle, and in interphase nuclei preferentially forms a perinucleolar aggregate in the proximity of a subset of NBL2 loci. TNBL aggregates interact with the SAM68 perinucleolar body in a mirror-image cancer specific perinucleolar structure. TNBL binds with high affinity to several proteins involved in nuclear functions and RNA metabolism, such as CELF1 and NPM1. Our data unveil novel DNA and RNA structural features of a non-coding macrosatellite frequently altered in cancer.
Note: Reproducció del document publicat a: https://doi.org/10.1093/nar/gky263
It is part of: Nucleic Acids Research, 2018, vol. 46, num. 11, p. 5504-5524
URI: http://hdl.handle.net/2445/177771
Related resource: https://doi.org/10.1093/nar/gky263
ISSN: 0305-1048
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)

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