Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/178609
Title: | Gene expression analyses determine two different subpopulations in KIT-negative GIST-like (KNGL) patients |
Author: | Moura, David S. Ramos, Rafel Fernandez-Serra, Antonio Serrano Piñol, M. Teresa Cruz, Julia Alvarez-Alegret, Ramiro Ortiz-Duran, Rosa Vicioso, Luis Gomez-Dorronsoro, Maria Luisa García del Muro Solans, Xavier Martinez-Trufero, Javier Rubió Casadevall, Jordi Sevilla, Isabel Lainez, Nuria Gutiérrez, Antonio Serrano, César Lopez-Alvarez, Maria Hindi, Nadia Taron, Miquel López Guerrero, José Antonio Martin-Broto, Javier |
Keywords: | RNA Expressió gènica Pacients RNA Gene expression Patients |
Issue Date: | 3-Apr-2018 |
Publisher: | Impact Journals |
Abstract: | Introduction: there are limited findings available on KIT-negative GIST-like (KNGL) population. Also, KIT expression may be post-transcriptionally regulated by miRNA221 and miRNA222. Hence, the aim of this study is to characterize KNGL population, by differential gene expression, and to analyze miRNA221/222 expression and their prognostic value in KNGL patients. Methods: KIT, PDGFRA, DOG1, IGF1R, MIR221 and MIR222 expression levels were determined by qRT-PCR. We also analyzed KIT and PDGFRA mutations, DOG1 expression, by immunohistochemistry, along with clinical and pathological data. Disease-free survival (DFS) and overall survival (OS) differences were calculated using Log-rank test. Results: hierarchical cluster analyses from gene expression data identified two groups: group I had KIT, DOG1 and PDGFRA overexpression and IGF1R underexpression and group II had overexpression of IGF1R and low expression of KIT, DOG1 and PDGFRA. Group II had a significant worse OS (p = 0.013) in all the series, and showed a tendency for worse OS (p = 0.11), when analyzed only the localized cases. MiRNA222 expression was significantly lower in a control subset of KIT-positive GIST (p < 0.001). OS was significantly worse in KNGL cases with higher expression of MIR221 (p = 0.028) or MIR222 (p = 0.014). Conclusions: we identified two distinct KNGL subsets, with a different prognostic value. Increased levels of miRNA221/222, which are associated with worse OS, could explain the absence of KIT protein expression of most KNGL tumors. |
Note: | Reproducció del document publicat a: https://doi.org/10.18632/oncotarget.24799 |
It is part of: | Oncotarget, 2018, vol. 9, num. 25, p. 17576-17588 |
URI: | http://hdl.handle.net/2445/178609 |
Related resource: | https://doi.org/10.18632/oncotarget.24799 |
ISSN: | 1949-2553 |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
688187.pdf | 1.31 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License